The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.

WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  FC: 1/50-1/100   ELISA: 1/10000

Western

Western blot analysis of PTP1B in  Jurkat (1), MCF-7 (2) cell lysate using PTP1B antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human bladder cancer tissues using PTP1B antibody.

Immunocytochemistry

Immunocytochemistry analysis of PTP1B in  Hela cells using PTP1B antibody (green),and DAPI(blue). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow

Flow Cytometry analysis of  Jurkat cells using PTP1B antibody (green) and negative control (red).

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