Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

推荐稀释比 WB:1/1000-1/2000; IHC:1/100-1/200; IF:1/50-1/200

Western blot analysis on 2 NIH/3T3 lysates treated with PDGF using Phospho-AKT (Ser473) antibody.

Immunohistochemistry analysis of paraffin-embedded Human kidney using Phospho-Akt(Ser473) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of Phospho-AKT (Ser473) in NIH/3T3 treated with PDGF using Phospho-Akt(Ser473) antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.