操作手册
产品名称 |
产品编号 |
规格 |
Hieff NGS® G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina® CUT&Tag试剂盒(pA/G-Tn5版) |
12598ES04 |
4 T |
12598ES12 |
12 T |
|
12598ES48 |
48 T |
组分信息
组分编号 |
组分名称 |
12598ES04 |
12598ES12 |
12598ES48 |
||
BOX-I |
12598-A |
|
ConA Beads |
40 μL |
120 μL |
480 μL |
12598-B |
|
DNA Selection Beads |
400 μL |
1.2 mL |
4.8 mL |
|
12598-C |
|
Cell wash buffer |
3.6 mL |
10.8 mL |
43.2 mL |
|
12598-D |
|
ConA bind buffer |
400 μL |
1.2 mL |
4.8 mL |
|
BOX-II |
12598-E |
|
Protease Inhibitor Cocktail (EDTA-free) |
40 μL |
120 μL |
480 μL |
12598-F |
|
5% Digitonin |
15 μL |
45 μL |
180 μL |
|
12598-G |
|
3 M NaCl |
80 μL |
240 μL |
960 μL |
|
12598-H |
|
50x Protein blocker |
4 μL |
12 μL |
48 μL |
|
12598-I |
|
pA/G-Transposome Mix |
4 μL |
12 μL |
48 μL |
|
12598-J |
|
30x Activating buffer |
4 μL |
12 μL |
48 μL |
|
12598-K |
|
15x Terminate Solution |
8 μL |
24 μL |
96 μL |
|
12598-L |
|
DNA Spike-in mix (5 pg/uL) |
4 μL |
12 μL |
48 μL |
|
12598-M |
|
30x Proteinase K |
4 μL |
12 μL |
48 μL |
|
12598-N |
|
Primer Mix |
12 μL |
36 μL |
144 μL |
|
12598-O |
|
2×Ultima Amplification Mix |
100 μL |
300 μL |
1.2 mL |
|
12598-P |
|
N5(N501) * |
4 μL |
NA |
NA |
|
12598-Q |
|
N7(N701) * |
4 μL |
NA |
NA |
【注】:*测序时 Index 序列 填写时,测序平台为NovaSeq 6000 v1.0 reagents, MiSeq, HiSeq 2000/2500,则N501-CTCTCTAT,N701-TAAGGCGA。测序平台为NovaSeq 6000 v1.5 reagents,MiniSeq, NextSeq,HiSeq 3000/4000,则N501-ATAGAGAG,N701-TAAGGCGA。
BOX-I:2-8℃保存,BOX-II:-25~-15℃保存,有效期1年。
CN20231208