Recombinant Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein,hFc Tag

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产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统(Source

Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp108(CD3E)&Phe22-Ala105(CD3D).

[Accession | P22646(CD3E)&P04235(CD3D)]

分子量大小(Molecular Weight

The protein has a predicted MW of 36.1 kDa (CD3E) and 35.2 kDa (CD3D). Due to glycosylation,the protein migrates to 50-65 kDa and 40-50 kDa based on SDS- PAGE result.

内毒素(Endotoxin

<1EU per μg by the LAL method.

纯度(Purity

> 95% as determined by SDS- PAGE and HPLC

制剂(Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.        

 

Ver. CN20230625

400-6111-883