性能参数
表达区间及表达系统(Source) |
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp108(CD3E)&Phe22-Ala105(CD3D). [Accession | P22646(CD3E)&P04235(CD3D)] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 36.1 kDa (CD3E) and 35.2 kDa (CD3D). Due to glycosylation,the protein migrates to 50-65 kDa and 40-50 kDa based on SDS- PAGE result. |
内毒素(Endotoxin) |
<1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS- PAGE and HPLC |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230625
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