Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Western

Western blot analysis of Phospho-AKT (Thr450) in A549 lysates untreated or treated with EGF lysates using AKT (Phospho-Thr450) antibody(upper) and AKT(pan) antibody(200323, lower).

Western

Western blot analysis of Phospho-AKT (Thr450) in 293 cells untreated or treated with EGF using AKT1 (Phospho-Thr450) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using AKT1 (Phospho-Thr45) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Phospho-AKT (Thr450) in Hela cells using AKT1 (Phospho-Thr450) antibody.