产品说明书
FAQ
COA
已发表文献
性能参数
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分子别名（Synonyms） |
HLAE;MHC HLA-E alpha-1;sHLA-E;HLAE |
|
表达区间及表达系统（Source） |
Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.[Accession | P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on SDS-PAGE result. |
|
内毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性（Activity） |
SPR Data:Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay .Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay . |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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重构方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
