Recombinant Human CD27 Ligand/CD70 Trimer Protein,His Tag

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产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统(Source

Recombinant Human CD27 Ligand/CD70 Trimer Protein is expressed from HEK293 with His tag at the N-terminal. It contains Leu50-Pro193.[Accession | P32970-1]

分子量大小(Molecular Weight

The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-65 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin

Less than 1EU per μg by the LAL method.

纯度(Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

活性(Activity

ELISA Data: Immobilized Human CD27 Ligand Trimer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA. 

制剂(Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.        

 Ver. CN20230602

400-6111-883