CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. Human and mouse CD27L share approximated 56% aa sequence identity. CD27L is expected to exist as non-covalent homotrimers. CD70 is constitutively expressed on medullary thymic epithelial cells (mTECs) in human and mouse. CD70 is also exclusively expressed on DCs, B cells, conventional- and regulatory T cells and NK cells but only after their activation.

 

产品性质

Synonyms	CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G
Uniprot No.	P32970-1
Source	Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Leu50-Pro193.
Molecular Weight	The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% as determined by SDS-PAGE and HPLC
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

The products are shipped with ice pack and can be stored at -20℃ for 1 year.

2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 -6 months, -20 to -80°C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

 

注意事项

1.For your safety and health, please wear lab coats and disposable gloves for operation.

2.For research use only!