Recombinant Human Glypican 1/GPC1 Protein,His Tag

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产品说明书

FAQ

COA

已发表文献

 

Glypican 1, also known as GPC1, is a cell surface heparan sulfate proteoglycan that plays a crucial role in cell growth and development. It is a member of the glypican family, which are glycosylphosphatidylinositol-anchored proteins found on the cell surface.

 

性能参数

Synonyms

Glypican 1, GPC1

Uniprot No.

P35052-1

Source

Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp24-Ser530.

Molecular Weight

The protein has a predicted MW of 57.17 kDa. And it migrates as 60-72 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

> 90% as determined by SDS-PAGE.

Biological Activity

Human FGF-2 immobilized on CM5 chip can bind human GPC1, His tag, with an affinity constant of 5.83 × 10-9 M as determined in a SPR assay (Biacore 8K).

Endotoxin

< 1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS.

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

 

注意事项

1.Please operate with lab coats and disposable gloves, for your safety.

2.This product is for research use only.

 

产品数据

Ver. CN20230831

400-6111-883