产品说明书
FAQ
COA
已发表文献
性能参数
|
表达区间及表达系统（Source） |
Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide. [Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
|
内毒素（Endotoxin） |
< 1EU /μg by the LAL method. |
|
纯度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M &NY-ESO-1(SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA . SPR Data: Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay |
|
制剂（Formulation） |
Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 7.4). |
储存条件
The product should be stored at -85~-65℃ for 12 months from date of receipt.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230607
