Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

推荐稀释比 WB:1/500-1/1000

Western blot analysis of Phospho-IRE1 (S724) in (1) K562 lysates treated with AP; (2) Untreated K562 lysates using Phospho-IRE1 (Ser724) antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.