Q：为什么柱子反压过高？

A：可能是填料被堵塞；裂解液中含有微笑的固体颗粒，建议上柱前使用 0.22 µm/0.45

µm 滤膜过滤。

Q：为什么洗脱组分中没有目的蛋白？

A：可能是抗体被降解；建议适当的提高洗脱pH。

Q：该产品可以直接使用吗？

A：Protein A 琼脂糖珠最好在使用前充分颠倒若干次，使琼脂糖珠混合均匀。

Q：该产品可以贮存在-20℃吗？

A：不可以，不可冷冻该产品。应置于 4°贮存。

Q：说明书中COIP实验操作中取适量填料是要加多少量的填料？

A：一般填料的量是根据自己要做的样品的量决定。最小的常用COIP体系为：20ul填料+5ug抗体与总体积500ul 总蛋白浓度1mg/ml的裂解液混合孵育。

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