磷酸缓冲盐溶液(PBS,细胞培养级Phosphate-Buffered Saline)是最常用的缓冲溶液之一,主要由NaCl、KCl、Na2HPO4、KH2PO4组成,不含Ca2+、Mg2+,pH值为7.2-7.4,该试剂经过滤除菌,常用于细胞培养过程中细胞的洗涤或其他常规用途。该试剂为1×工作液,可以直接使用。
| 产品货号 | 60158ES | 41403ES | 41404ES | 60154ES | 60152ES | 60173ES |
| 产品名称 | PBS (1×) | PBS (1×) | PBS (10×) | D-PBS (1×) | D-PBS (1×) | D-PBS (1×) |
| 产品形式 | 粉末(袋装即溶形式) | 液体(即用型) | 粉末(袋装即溶形式) | 液体(即用型) | ||
| 产品优点 | 粉末款:成本友好,自配灭菌,灵活掌控 液体款:开瓶即用,省时省力,实验高效 | |||||
| 产品特点 | 无钙镁离子 | 含钙镁离子 | ||||
| NaCl | 137.07 mM | 137 mM | 1.37 M | 137.93 mM | 137.93 mM | 137.93 mM |
| KCl | 2.67 mM | 2.67 mM | 27 mM | 2.67 mM | 2.67 mM | 2.67 mM |
| Na2HPO4 | 10 mM | 10 mM | 100 mM | 8.07 mM | 8.06 mM | 8.06 mM |
| KH2PO4 | 1.84 mM | 2 mM | 18 mM | 1.47 mM | 1.47 mM | 1.47 mM |
| CaCl2 | / | / | / | / | / | 0.90 mM |
| MgCl2-6H2O | / | / | / | / | / | 0.49 mM |
| D-Glucose | / | / | / | / | / | 5.55 mM |
| Sodium Pyruvate | / | / | / | / | / | 0.33 mM |
注:
1.消化细胞时,推荐使用不含钙镁离子的PBS/D-PBS,清洗掉螯合剂,保证消化效果。
2.D-PBS的磷酸盐浓度通常略低,以更接近生理渗透压,更适合细胞洗涤、细胞稀释计数、细胞保存和细胞培养。
3.60173ES更适合细胞培养,其中Ca2+和Mg2+有助于保持细胞附着,D-Glucose和Sodium Pyruvate为细胞提供能量来源和支持细胞存活。
4.具体使用情况请结合文献或者对应产品推荐,合理选择PBS/D-PBS。
产品组分
| 组分 | 浓度 |
| NaCl | 137 mM |
| KCl | 2.67 mM |
| Na2HPO4 | 10 mM |
| KH2PO4 | 2 mM |
常温运输,2~8℃或室温保存,2年有效。
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