产品说明书
FAQ
COA
已发表文献
性能参数
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表达区间及表达系统（Source） |
Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln31-Gln692.[Accession | Q8NBP7-1] |
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分子量大小（Molecular Weight） |
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result. |
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内毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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纯度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA . SPR Data :Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). |
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制剂（Formulation） |
Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4). |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230721
