本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:
1)快速,仅需 1-5 min即可完成连接反应;
2)高效,无自连,阳性克隆率接近100%,无需设置蓝白斑筛选;
3)操作简单,从连接到涂板仅需 15-20 min。操作过程省去冰浴、热激和1 h复苏;
4)可连接长达5 kb目的产物;
5)Hieff Clone® Zero TOPO-TA Simple Cloning Kit不含多克隆酶切位点(MCS),在引入后续酶切位点进行酶切操作时,不会受到MCS位点的影响,从而增加酶切效率以及克隆成功率。
- 快速、高效、操作简单、后续不受MCS位点影响
冰袋运输。-20℃保存,避免反复冻融。有效期一年。
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[2] Zhang W, Sun X, Wei X, Gao Y, Song J, Bai Y. Geography-Driven Evolution of Potato Virus A Revealed by Genetic Diversity Analysis of the Complete Genome. Front Microbiol. 2021;12:738646. Published 2021 Oct 1. doi:10.3389/fmicb.2021.738646(IF:5.640)
[3] Papolu PK, Ramakrishnan M, Wei Q, et al. Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress. BMC Plant Biol. 2021;21(1):585. Published 2021 Dec 9. doi:10.1186/s12870-021-03339-1(IF:4.215)
[4] Fang Y, Tambo E, Xue JB, Zhang Y, Zhou XN, Khater EIM. Molecular Analysis of Targeted Insecticide Resistance Gene Mutations in Field-Caught Mosquitos of Medical Importance From Saudi Arabia. J Med Entomol. 2021;58(4):1839-1848. doi:10.1093/jme/tjab048(IF:2.278)
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