零背景TOPO-Blunt平末端克隆试剂盒|Zero TOPO-Blunt Simple Cloning Kit

Hieff Clone® Zero TOPO-Blunt Simple Cloning Kit 零背景TOPO-Blunt平末端克隆试剂盒（不含MCS多克隆酶切位点）

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已发表文献

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产品介绍

本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：

1）快速，仅需1-5分钟即可完成连接反应；

2）高效，无自连现象，阳性克隆率接近100%，无需设置蓝白斑筛选；

3）操作简单，从连接到涂板仅需15-20 min。操作过程省去冰浴、热激和1 h复苏；

4）可连接长达5 kb目的产物；

5）Hieff Clone® Topo-blunt simple vector不含多克隆酶切位点（MCS），使用者在引入后续酶切位点进行酶切操作时，不会受到MCS位点的影响，从而增加酶切效率以及克隆成功率。

产品特色

快速、高效、操作简单、后续不受MCS位点影响

存储条件

冰袋运输。-20℃保存，避免反复冻融。有效期一年。

COA

已发表文献

[ 1 ]The oriental armyworm genome yields insights into the long-distance migration of noctuid moths

Journal:Cell Reports|DOI:10.1016/j.celrep.2022.111843|IF:10

[ 2 ]Volatile signaling in plant–Metarhizium–insect interactions: Implications for nitrogen cycling

Journal:PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA|DOI:10.1073/pnas.2603282123|IF:9.5

[ 3 ]Chromosome-level genome reference and genome editing of the tea geometrid

Journal:Molecular Ecology Resources|DOI:10.1111/1755-0998.13385|IF:7.09

[ 4 ]Genome-wide analysis of V-ATPase genes in Plutella xylostella (L.) and the potential role of PxVHA-G1 in resistance to Bacillus thuringiensis Cry1Ac toxin

Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES|DOI:10.1016/j.ijbiomac.2021.11.169|IF:6.95

[ 5 ]A complement factor I (CFI) gene mediates innate immune responses in yellow catfish Pelteobagrus fulvidraco

Journal:GENOMICS|DOI:10.1016/j.ygeno.2020.09.034|IF:6.21

[ 6 ]The Potential Role of the Methionine Aminopeptidase Gene PxMetAP1 in a Cosmopolitan Pest for Bacillus thuringiensis Toxin Tolerance

Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES|DOI:10.3390/ijms232113005|IF:6.21

[ 7 ]Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES|DOI:10.3390/ijms24021730|IF:5.6

[ 8 ]CRISPR/Cas9-mediated knockout of LW-opsin reduces the efficiency of phototaxis in the diamondback moth Plutella xylostella

Journal:PEST MANAGEMENT SCIENCE|DOI:10.1002/ps.6405|IF:4.85

[ 9 ]Bioactivity and mitochondrial mode of action of methyl benzoate against the tea pest Ectropis grisescens

Journal:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY|DOI:10.1016/j.pestbp.2026.107153|IF:4.8

[ 10 ]Overcoming Target Drift: Development and Validation of a One-Step TaqMan qPCR Assay for Epidemiological Surveillance of Carpione rhabdovirus Circulating in Southern China

Journal:Microorganisms|DOI:10.3390/microorganisms14010126|IF:4.7

[ 11 ]Molecular characterization and overexpression of the difenoconazole resistance gene CYP51 in Lasiodiplodia theobromae field isolates

Journal:Scientific Reports|DOI:10.1038/s41598-021-03601-4|IF:4.38

[ 12 ]Long Noncoding RNA Lnc-MxA Inhibits Beta Interferon Transcription by Forming RNA-DNA Triplexes at Its Promoter

Journal:JOURNAL OF VIROLOGY|DOI:10.1128/JVI.00786-19|IF:4.32

[ 13 ]Molecular characterization of hypoxia-inducible factor alpha isoforms reveals a pivotal role of HIF-3α in acute hypoxia adaptation of greenfin horse-faced filefish (Thamnaconus septentrionalis)

Journal:Frontiers in Marine Science|DOI:10.3389/fmars.2026.1816646|IF:3.4

[ 14 ]Functional identification of C-type lectin in the diamondback moth, Plutella xylostella (L.) innate immunity

Journal:Journal of Integrative Agriculture|DOI:10.1016/S2095-3119(21)63650-X|IF:2.85

[ 15 ]UDP-Glucosyltransferases Induced by Nosema bombycis Provide Resistance to Microsporidia in Silkworm (Bombyx mori)

Journal:Insects|DOI:10.3390/insects12090799|IF:2.77

[ 16 ]Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in Plutella xylostella

Journal:Insects|DOI:10.3390/insects12080712|IF:2.77

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