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产品货号 | 产品名称 | 同裂酶 | 酶活 (U/uL) |
规格 (T) |
识别序列 | 反应温度 (ºC) |
热失活方式 | 甲基化影响 |
15001ES50 | FuniCut® AscI | PalAI, SgsI | 10 | 50T | 5'-GG↓CGCGCC-3' 3'-CCGCGC↑GG-5' |
37℃ | 80℃, 20 min | CpG |
15002ES25 | FuniCut® AvrII | AspA2I, BlnI, XmaJI | 5 | 25T | 5'-C↓CTAGG-3' 3'-GGATC↑C-5' |
37℃ | 80℃, 20 min | 无影响 |
15003ES76 | FuniCut® BamHI | 无 | 20 | 500T | 5'-G↓GATCC-3' 3'-CCTAG↑G-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | 无影响 |
15004ES62 | FuniCut® BclI | BsiQI, FbaI, Ksp22I | 20 | 125T | 5'-T↓GATCA-3' 3'-ACTAG↑T-5' |
37℃ | 80℃, 20 min | Dam;EcoBI |
15005ES50 | FuniCut® BsaI | 无 | 20 | 50T | 5'-GGTCTC(N)1↓-3' 3'-CCAGAG(N)5↑-5' |
37℃ | 80℃, 20 min | Dcm;CpG |
15006ES60 | FuniCut® BstEII | BstPI,Eco91I,EcoO65I,PspEI | 20 | 100T | 5'-G↓GTNACC-3' 3'-CCANTG↑G-5' |
37℃ | 80℃, 20 min | 无影响 |
15007ES50 | FuniCut® ClaI | BspDI,BanIII,Bsa29I,BseCI,BshVI,BsiXI,Bsp106I,BspXI,Bsu15I,BsuTUI,ZhoI | 10 | 50T | 5'-AT↓CGAT-3' 3'-TAGC↑TA-5 |
37℃ | 80℃, 20 min | Dam;CpG |
15008ES50 | FuniCut® DpnII | BfuCI,MboI,Sau3AI,BscFI,Bsp143I,BssMI,BstENII,BstMBI,Kzo9I,NdeII | 50 | 50T | 5'-↓GATC-3' 3'-CTAG↑-5' |
37℃ | 80℃, 20 min | Dam;EcoBI |
15009ES25 | FuniCut® EagI | BseX3I, BstZI, EclXI, Eco52I, XmaIII | 20 | 25T | 5'-C↓GGCCG-3' 3'-GCCGG↑C-5' |
37℃ | 80℃, 20 min | CpG |
15010ES78 | FuniCut® EcoRI | FunII | 20 | 600T | 5'-G↓AATTC-3' 3'-CTTAA↑G-5' |
37℃ | 80℃, 20 min | CpG |
15011ES70 | FuniCut® EcoRV | Eco32I | 20 | 200T | 5'-GAT↓ATC-3' 3'-CTA↑TAG-5' |
37℃ | 80℃, 20 min | CpG |
15012ES76 | FuniCut® HindIII | 无 | 100 | 500T | 5'-A↓AGCTT-3' 3'-TTCGA↑A-5' |
37℃ | 80℃, 20 min | EcoBI |
15013ES50 | FuniCut® HpaI | BstHPI, KspAI | 5 | 50T | 5'-GTT↓AAC-3' 3'-CAA↑TTG-5' |
37℃ | 80℃, 20 min | CpG;EcoK |
15015ES70 | FuniCut® KpnI | 无 | 100 | 200T | 5'-GGTAC↓C-3' 3'-C↑CATGG-5' |
37℃ | 80℃, 20 min | 无影响 |
15016ES60 | FuniCut® MluI | 无 | 20 | 100T | 5'-A↓CGCGT-3' 3'-TGCGC↑A-5' |
37℃ | 80℃, 20 min | CpG |
15018ES30 | FuniCut® NcoI | Bsp19I | 20 | 30T | 5'-C↓CATGG-3' 3'-GGTAC↑C-5' |
37℃ | 80℃, 20 min | 无影响 |
15019ES70 | FuniCut® NdeI | FauNDI | 20 | 200T | 5'-CA↓TATG-3' 3'-GTAT↑AC-5' |
37℃ | 80℃, 20 min | 无影响 |
15020ES30 | FuniCut® NheI | AsuNHI | 20 | 30T | 5'-G↓CTAGC-3' 3'-CGATC↑G-5 |
37℃ | 80℃, 20 min | CpG |
15021ES50 | FuniCut® NotI | CciNI | 20 | 50T | 5'-GC↓GGCCGC-3' 3'-CGCCGG↑CG-5' |
37℃ | 80℃, 20 min | CpG |
15022ES76 | FuniCut® PstI | BspMAI | 20 | 500T | 5'-CTGCA↓G-3' 3'-G↑ACGTC-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | 无影响 |
15023ES60 | FuniCut® SacI | Psp124BI, SstI | 20 | 100T | 5'-GAGCT↓C-3' 3'-C↑TCGAG-5' |
37℃ | 80℃, 20 min | 无影响 |
15024ES70 | FuniCut® SalI | 无 | 20 | 200T | 5'-G↓TCGAC-3' 3'-CAGCT↑G-5' |
37℃ | 80℃, 20 min | CpG |
15025ES25 | FuniCut® SbfI | SdaI, Sse8387I | 20 | 25T | 5'-CCTGCA↓GG-3' 3'-GG↑ACGTCC-5' |
37℃ | 80℃, 20 min | 无影响 |
15027ES60 | FuniCut® SmaI | 无 | 20 | 100T | 5'-CCC↓GGG-3' 3'-GGG↑CCC-5' |
25℃ | 80℃, 20 min | CpG |
15028ES50 | FuniCut® Spel | AhlI, BcuI | 20 | 50T | 5'-A↓CTAGT-3' 3'-TGATC↑A-5' |
37℃ | 80℃, 20 min | EcoBI;EcoK |
15029ES50 | FuniCut® SphI | BbuI, PaeI, SpaHI | 20 | 50T | 5'-GCATG↓C-3' 3'-C↑GTACG-5' |
37℃ | 80℃, 20 min | EcoBI |
15030ES56 | FuniCut® SspI | 无 | 20 | 60T | 5'-AAT↓ATT-3' 3'-TTA↑TAA-5' |
37℃ | 80℃, 20 min | 无影响 |
15031ES60 | FuniCut® StuI | AatI, Eco147I, PceI, SseBI | 10 | 100T | 5'-AGG↓CCT-3' 3'-TCC↑GGA-5' |
37℃ | 80℃, 20 min | Dcm;EcoBI |
15032ES70 | FuniCut® TaqI | TthHB8I | 20 | 200T | 5'-T↓CGA-3' 3'-AGC↑T-5' |
65℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | Dam |
15033ES76 | FuniCut® XbaI | 无 | 20 | 500T | 5'-T↓CTAGA-3' 3'-AGATC↑T-5' |
37℃ | 80℃, 20 min | Dam |
15034ES76 | FuniCut® XhoI | PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI | 20 | 500T | 5'-C↓TCGAG-3' 3'-GAGCT↑C-5' |
37℃ | 80℃, 20 min | CpG |
15036ES50 | FuniCut® FspI | Acc16I, Avill, MstI, NsbI | 10 | 50T | 5'-TGC↓GCA-3' 3'-ACG↑CGT-5' |
37℃ | 80℃, 20 min | CpG |
15038ES76 | FuniCut® HinfI | 无 | 50 | 500T | 5'-G↓ANTC-3' 3'-CTNA↑G-5 |
37℃ | 80℃, 20 min | CpG;EcoBI |
15039ES70 | FuniCut® ApaLI | 无 | 10 | 200T | 5'-G↓TGCAC-3' 3'-CACGT↑G-5' |
37℃ | 80℃, 20 min | CpG |
15040ES50 | FuniCut® NruI | 无 | 20 | 50T | 5'-TCG↓CGA-3' 3'-AGC↑GCT-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | CpG;Dam |
15041ES25 | FuniCut® PacI | 无 | 10 | 25T | 5'-TTAAT↓TAA-3' 3'-AAT↑TAATT-5' |
37℃ | 80℃, 20 min | EcoK |
15042ES70 | FuniCut® PvuII | 无 | 20 | 200T | 5'-CAG↓CTG-3' 3'-GTC↑GAC-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | EcoBI |
15043ES50 | FuniCut® SacII | Cfr42I,KspI,Sfr303I,SgrBI | 20 | 50T | 5'-CCGC↓GG-3' 3'-GG↑CGCC-5' |
37℃ | 80℃, 20 min | CpG |
15044ES25 | FuniCut® NsiI | EcoT22I, Mph1103I, Zsp2I | 20 | 25T | 5'-ATGCA↓T-3' 3'-T↑ACGTA-5' |
37℃ | 80℃, 20 min | EcoBI |
15048ES30 | FuniCut® Esp3I (BsmBI) | BsmBI, BstGZ53I, Esp16I, Esp23I | 10 | 30T | 5'-CGTCTC(N)1↓-3' 3'-GCAGAG(N)5↑-5' |
37℃ | 80℃, 20 min | CpG;EcoBI |
15049ES60 | FuniCut® BglII | 无 | 50 | 100T | 5'-A↓GATCT-3' 3'-TCTAG↑A-5' |
37℃ | 80℃, 20 min | EcoBI |
15050ES60 | FuniCut® BstBI | AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI | 20 | 100T | 5'-TT↓CGAA-3' 3'-AAGC↑TT-5' |
37℃ | 80℃, 20 min | CpG |
15051ES50 | FuniCut® MnlI | 无 | 5 | 50T | 5'-CCTCN7↓-3' 3'-GGAGN6↑-5' |
37℃ | 80℃, 20 min | EcoBI |
15052ES50 | FuniCut® DpnI | 无 | 20 | 50T | 5'-GAm6↓TC-3' 3'-CT↑Am6G-5' |
37℃ | 80℃, 20 min | CpG |
15056ES25 | FuniCut® AgeI | AsiGI, CspAI, BshTI, PinAI | 20 | 25 T | 5'-A↓CCGGT-3' 3'-TGGCC↑A-5' |
37℃ | 80℃, 20 min | CpG |
15057ES60 | FuniCut® SfiI | 无 | 20 | 100 T | 5'-GGCCN4↓NGGCC-3' 3'-CCGGN↑N4CCGG-5' |
50℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | Dcm;CpG |
15058ES70 | FuniCut® PspGI | EcoRII, MvaI, BstNI, BciT130I, BseBI, AjnI, Psp6I, Bst2UI | 10 | 200 T | 5'-↑CCWGG-3' 3'-GGWCC↑-5' |
75℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | Dcm |
15059ES60 | FuniCut® DraI | 无 | 5 | 100 T | 5'-TTT↓AAA-3' 3'-AAA↑TTT-5' |
37℃ | 80℃, 20 min | EcoK |
15060ES56 | FuniCut® XcmI | XcmI、Xcm I、Xcm i、Xcm1、Xcm 1、XcmⅠ、Xcm Ⅰ、Xcm | 5 | 60 T | 5'-CCA(N)5↓(N)4TGG-3' 3'-GGT(N)4↑(N)5ACC-5' |
37℃ | 80℃, 20 min | 无影响 |
15061ES30 | FuniCut® BsrGI | BstAUI, Bsp1407I | 20 | 30 T | 5'-T↓GTACA-3' 3'-ACATG↑T-5' |
37℃ | 80℃, 20 min | 无影响 |
15062ES50 | FuniCut® PmeI | MssI | 20 | 50 T | 5'-GTTT↓AAAC-3' 3'-CAAA↑TTTG-5' |
37℃ | 80℃, 20 min | CpG |
15063ES70 | FuniCut® HpaII | MspI, HapII, BsiSI | 20 | 200 T | 5'-C↓CGG-3' 3'-GGC↑C-5' |
37℃ | 80℃, 20 min | CpG |
15064ES72 | FuniCut® MspI | HpaII, HapII, BsiSI | 10 | 250 T | 5'-C↓CGG-3' 3'-GGC↑C-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | 无影响 |
15065ES50 | FuniCut® AccI | XmiI, FblI | 10 | 50 T | 5'-GT↓MKAC-3' 3'-CAKM↑TG-5' |
37℃ | 80℃, 20 min | CpG |
15066ES30 | FuniCut® EarI | Eam1104I, Bst6I | 20 | 30 T | 5'-CTCTTC(N)₁↓-3' 3'- GAGAAG(N)₄↑-5' |
37℃ | 80℃, 20 min | CpG |
15067ES75 | FuniCut® HaeIII | BsuRI, BshFI, BspANI, BsnI | 10 | 300 T | 5'-GG↓CC-3' 3'-CC↑GG-5' |
37℃ | 80℃, 20 min | 无影响 |
15068ES25 | FuniCut® MunI (MfeI) | MfeI | 20 | 25 T | 5'-C↓AATTG-3' 3'-GTTAA↑C-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | 无影响 |
15069ES30 | FuniCut® PvuI | Ple19I | 20 | 30 T | 5'-CG AT↓CG-3' 3'-GC↑TAGC-5' |
37℃ | 80℃, 20 min | CpG |
15070ES25 | FuniCut® BbsI | BstV2I, BpiI | 20 | 25 T | 5'-GAAGAC(N)₂↓-3' 3'-CTTCTG(N)₆↑ |
37℃ | 80℃, 20 min | 无影响 |
15071ES50 | FuniCut® DdeI | BstDEI, HpyF3I | 10 | 50 T | 5'-C↓TNAG-3' 3'-GANT↑C-5' |
37℃ | 80℃, 20 min | EcoBI |
15072ES60 | FuniCut® HhaI | HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI | 20 | 100 T | 5'-GCG↓C-3' 3'-C↑GCG-5' |
37℃ | 80℃, 20 min | CpG |
15073ES60 | FuniCut® HinP1I | HhaI, CfoI, AspLEI, Hin6I, HspAI, BstHHI | 10 | 100 T | 5'-G↓CGC-3' 3'-CGC↑G-5' |
37℃ | 80℃, 20 min | CpG |
15074ES30 | FuniCut® DraIII | AdeI | 20 | 30 T | 5'-CAC(N)3↓GTG-3' 3'-GTG↑(N)3CAC-5' |
37℃ | 不可热失活,请使用酚氯仿抽提或柱纯化 | CpG;EcoKI;EcoBI |
15075ES70 | FuniCut® BglI | 无 | 10 | 200 T | 5'-GCC(N)4↓NGGC-3' 3'-CGGN↑(N)4CCG-5' |
37℃ | 80℃, 20 min | CpG |
15202ES | BspQI(10 U/μL) | 无 | 10 |
500 U 2500 U 10000 U |
5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5' |
50℃ | 80℃, 20 min | 无影响 |
15203ES | BsmBI(10 U/μL) | 无 | 10 | 1000 U | 5'-CGTCTC(N)1↓-3' 3'-GCAGAG(N)5↑-5' |
55℃ | 80℃, 20 min | CpG;EcoBI |
15210ES | Nt.BspQI(10 U/μL) | 无 | 10 | 2000 U | 5'-GCTCTTCN-3' 3'-CGAGAAGN-5' |
50℃ | 80℃, 20 min | 无影响 |
15211ES | BsiWI(10 U/μL) | PspLI, Pfl23II | 10 | 300 U | 5'-C↓GTACG-3' 3'-GCATG↑C-5' |
55℃ | 80℃, 20 min | CpG |