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限制性内切酶酶基本信息 |
限制性内切酶在各种buffer中的活性(%) |
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产品货号 |
产品名称 |
同裂酶 |
酶活 |
规格 |
识别序列 |
反应温度 |
热失活方式 |
甲基化影响 |
FuniCut™ |
Thermo Scientific |
NEB |
Takara |
15093ES30 |
ZraI |
20 |
30 T |
5'-GACGT↓C-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
< 25% |
100 |
< 25% |
|
15065ES50 |
XmiI, FblI |
10 |
50 T |
5'-GT↓MKAC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
<10 |
100 |
<10 |
|
15088ES25 |
BspACI, SsiI |
10 |
25 T |
5'-C↓CGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15081ES60 |
BspTI, BfrI, BstAFI, MspCI, Vha464I |
20 |
100 T |
5'-C↓TTAAG-3' |
37℃ |
80℃, 20 min |
/ |
100 |
75 |
100 |
100 |
|
15056ES25 |
AsiGI, CspAI, BshTI, PinAI |
20 |
25 T |
5'-A↓CCGGT-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
50 |
100 |
50 |
|
15078ES50 |
AluBI |
10 |
50 T |
5'-AG↓CT-3' |
37℃ |
80℃, 20 min |
CpG;EcoBI |
100 |
100 |
100 |
100 |
|
15085ES70 |
Bsp120I, PspOMI |
20 |
200 T |
5'-GGGCC↓C-3' |
37℃ |
80℃, 20 min |
Dcm;CpG |
100 |
<12.5% |
100 |
<12.5% |
|
15039ES70 |
无 |
10 |
200T |
5'-G↓TGCAC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
75 |
100 |
100 |
|
15001ES50 |
PalAI, SgsI |
10 |
50T |
5'-GG↓CGCGCC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15086ES60 |
PshBI, VspI |
10 |
100 T |
5'-ATTA↓AT-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15091ES60 |
Bme18I, Eco47I |
10 |
100 T |
5'-G↓GWCC-3' |
37℃ |
80℃, 20 min |
CpG;Dcm |
100 |
100 |
100 |
100 |
|
15002ES25 |
AspA2I, BlnI, XmaJI |
5 |
25T |
5'-C↓CTAGG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15003ES76 |
无 |
20 |
500T |
5'-G↓GATCC-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
无影响 |
100 |
100 |
100 |
100 |
|
15070ES25 |
BstV2I, BpiI |
20 |
25 T |
5'-GAAGAC(N)₂↓-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
<10 |
100 |
25 |
|
15004ES62 |
BsiQI, FbaI, Ksp22I |
20 |
125T |
5'-T↓GATCA-3' |
37℃ |
80℃, 20 min |
Dam;EcoBI |
100 |
100 |
100 |
100 |
|
15075ES70 |
无 |
10 |
200 T |
5'-GCC(N)4↓NGGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15049ES60 |
无 |
50 |
100T |
5'-A↓GATCT-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
50 |
|
15096ES50 |
FuniCut® Bsh1236I (BstUI) |
BstUI, BspFNI, BstFNI, AccII |
20 |
50T |
5'-CG↓CG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
15005ES50 |
无 |
20 |
50T |
5'-GGTCTC(N)1↓-3' |
37℃ |
80℃, 20 min |
Dcm;CpG |
100 |
100 |
100 |
100 |
|
15090ES50 |
BsaMI, Mva1269I, PctI |
10 |
50 T |
5'-GAATGCN↓-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15094ES30 |
CciI, PagI |
10 |
30 T |
5'-T↓CATGA-3' |
37℃ |
80℃, 20 min |
Dam;EcoBI |
100 |
100 |
100 |
100 |
|
15061ES30 |
BstAUI, Bsp1407I |
20 |
30 T |
5'-T↓GTACA-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15050ES60 |
AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI |
20 |
100T |
5'-TT↓CGAA-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15006ES60 |
BstPI,Eco91I,EcoO65I,PspEI |
20 |
100T |
5'-G↓GTNACC-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
75 |
100 |
75 |
|
15095ES56 |
SacII, KspI, Sfr303I, SgrBI |
20 |
60 T |
5'-CCGC↓GG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
< 12.5% |
100 |
< 12.5% |
|
15007ES50 |
BspDI,BanIII,Bsa29I,BseCI,BshVI,BsiXI,Bsp106I,BspXI,Bsu15I,BsuTUI,ZhoI |
10 |
50T |
5'-AT↓CGAT-3' |
37℃ |
80℃, 20 min |
Dam;CpG |
100 |
100 |
100 |
100 |
|
15071ES50 |
BstDEI, HpyF3I |
10 |
50 T |
5'-C↓TNAG-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
50 |
100 |
50 |
|
15052ES50 |
无 |
20 |
50T |
5'-GAm6↓TC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15008ES50 |
BfuCI,MboI,Sau3AI,BscFI,Bsp143I,BssMI,BstENII,BstMBI,Kzo9I,NdeII |
50 |
50T |
5'-↓GATC-3' |
37℃ |
80℃, 20 min |
Dam;EcoBI |
100 |
75 |
100 |
75 |
|
15059ES60 |
无 |
5 |
100 T |
5'-TTT↓AAA-3' |
37℃ |
80℃, 20 min |
EcoK |
100 |
100 |
100 |
100 |
|
15074ES30 |
AdeI |
20 |
30 T |
5'-CAC(N)3↓GTG-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
CpG;EcoKI;EcoBI |
100 |
100 |
100 |
100 |
|
15009ES25 |
BseX3I, BstZI, EclXI, Eco52I, XmaIII |
20 |
25T |
5'-C↓GGCCG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15066ES30 |
Eam1104I, Bst6I |
20 |
30 T |
5'-CTCTTC(N)₁↓-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
12.5 |
100 |
25 |
|
15010ES78 |
FunII |
20 |
600T |
5'-G↓AATTC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15011ES70 |
Eco32I |
20 |
200T |
5'-GAT↓ATC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15048ES30 |
BsmBI, BstGZ53I, Esp16I, Esp23I |
10 |
30T |
5'-CGTCTC(N)1↓-3' |
37℃ |
80℃, 20 min |
CpG;EcoBI |
100 |
100 |
100 |
100 |
|
15083ES60 |
BstF5I, BtsCI, BseGI |
5 |
100 T |
5'-GGATG(N)9↓-3' |
37℃ |
80℃, 20 min |
Dcm;CpG;EcoBI |
100 |
50 |
100 |
50 |
|
15036ES50 |
Acc16I, Avill, MstI, NsbI |
10 |
50T |
5'-TGC↓GCA-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15067ES75 |
BsuRI, BshFI, BspANI, BsnI |
10 |
300 T |
5'-GG↓CC-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15072ES60 |
HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
20 |
100 T |
5'-GCG↓C-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15012ES76 |
无 |
100 |
500T |
5'-A↓AGCTT-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
75 |
100 |
100 |
|
15038ES76 |
无 |
50 |
500T |
5'-G↓ANTC-3' |
37℃ |
80℃, 20 min |
CpG;EcoBI |
100 |
100 |
100 |
50 |
|
15073ES60 |
HhaI, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
10 |
100 T |
5'-G↓CGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
75 |
100 |
75 |
|
15013ES50 |
BstHPI, KspAI |
5 |
50T |
5'-GTT↓AAC-3' |
37℃ |
80℃, 20 min |
CpG;EcoK |
100 |
100 |
100 |
50 |
|
15063ES70 |
MspI, HapII, BsiSI |
20 |
200 T |
5'-C↓CGG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
<25 |
100 |
<25 |
|
15015ES70 |
无 |
100 |
200T |
5'-GGTAC↓C-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15082ES50 |
Bsp143I, Kzo9I, BssMI, DpnII, BstMBI, NdeII, Sau3AI |
5 |
50 T |
5'-↓GATC-3' |
37℃ |
80℃, 20 min |
Dam;CpG;EcoBI |
100 |
100 |
100 |
100 |
|
15016ES60 |
无 |
20 |
100T |
5'-A↓CGCGT-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
75 |
100 |
100 |
|
15051ES50 |
无 |
5 |
50T |
5'-CCTCN7↓-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15087ES30 |
BalI, MlsI, Mox20I, Msp20I |
5 |
30 T |
5'-TGG↓CCA-3' |
37℃ |
80℃, 20 min |
Dcm |
100 |
100 |
100 |
100 |
|
15079ES50 |
Tru1I, Tru9I, SaqAI |
10 |
50 T |
5'-T↓TAA-3' |
37℃ |
80℃, 20 min |
EcoKI |
100 |
25 |
100 |
25 |
|
15064ES72 |
HpaII, HapII, BsiSI |
10 |
250 T |
5'-C↓CGG-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
无影响 |
100 |
25 |
100 |
25 |
|
15068ES25 |
MfeI |
20 |
25 T |
5'-C↓AATTG-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
无影响 |
100 |
12.5 |
100 |
12.5 |
|
15018ES30 |
Bsp19I |
20 |
30T |
5'-C↓CATGG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15019ES70 |
FauNDI |
20 |
200T |
5'-CA↓TATG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15020ES30 |
AsuNHI |
20 |
30T |
5'-G↓CTAGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15021ES50 |
CciNI |
20 |
50T |
5'-GC↓GGCCGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15040ES50 |
无 |
20 |
50T |
5'-TCG↓CGA-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
CpG;Dam |
100 |
100 |
100 |
100 |
|
15044ES25 |
EcoT22I, Mph1103I, Zsp2I |
20 |
25T |
5'-ATGCA↓T-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15041ES25 |
无 |
10 |
25T |
5'-TTAAT↓TAA-3' |
37℃ |
80℃, 20 min |
EcoK |
100 |
100 |
100 |
100 |
|
15062ES50 |
MssI |
20 |
50 T |
5'-GTTT↓AAAC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
50 |
100 |
50 |
|
15092ES60 |
AcvI, Eco72I, PspCI, BbrPI |
20 |
100 T |
5'-CAC↓GTG-3' |
37℃ |
80℃, 20 min |
CpG;EcoKI;EcoBI |
100 |
< 12.5% |
100 |
< 12.5% |
|
15058ES70 |
EcoRII, MvaI, BstNI, BciT130I, BseBI, AjnI, Psp6I, Bst2UI |
10 |
200 T |
5'-↑CCWGG-3' |
75℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
Dcm |
100 |
100 |
100 |
50 |
|
15022ES76 |
BspMAI |
20 |
500T |
5'-CTGCA↓G-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
无影响 |
100 |
100 |
100 |
100 |
|
15069ES30 |
Ple19I |
20 |
30 T |
5'-CG AT↓CG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15042ES70 |
无 |
20 |
200T |
5'-CAG↓CTG-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
EcoBI |
100 |
100 |
100 |
100 |
|
15084ES60 |
AfaI, Csp6I, CviQI, RsaNI |
10 |
100 T |
5'-GT↓AC-3' |
37℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
CpG |
100 |
25 |
100 |
25 |
|
15023ES60 |
Psp124BI, SstI |
20 |
100T |
5'-GAGCT↓C-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15043ES50 |
Cfr42I,KspI,Sfr303I,SgrBI |
20 |
50T |
5'-CCGC↓GG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15024ES70 |
无 |
20 |
200T |
5'-G↓TCGAC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15025ES25 |
SdaI, Sse8387I |
20 |
25T |
5'-CCTGCA↓GG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
100 |
100 |
100 |
|
15089ES60 |
ZrmI, BmcAI |
20 |
100 T |
5'-AGT↓ACT-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15076ES25 |
SbfI, Sse8387I |
20 |
25 T |
5'-CCTGCA↓GG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
<12.5 |
100 |
<12.5 |
|
15057ES60 |
无 |
20 |
100 T |
5'-GGCCN4↓NGGCC-3' |
50℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
Dcm;CpG |
100 |
100 |
100 |
100 |
|
15027ES60 |
无 |
20 |
100T |
5'-CCC↓GGG-3' |
25℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15077ES56 |
Eco105I, BstSNI |
5 |
60 T |
5'-TAC↓GTA-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
50 |
100 |
50 |
|
15028ES50 |
AhlI, BcuI |
20 |
50T |
5'-A↓CTAGT-3' |
37℃ |
80℃, 20 min |
EcoBI;EcoK |
100 |
100 |
100 |
100 |
|
15029ES50 |
BbuI, PaeI, SpaHI |
20 |
50T |
5'-GCATG↓C-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
100 |
100 |
100 |
|
15030ES56 |
无 |
20 |
60T |
5'-AAT↓ATT-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
50 |
100 |
100 |
|
15031ES60 |
AatI, Eco147I, PceI, SseBI |
10 |
100T |
5'-AGG↓CCT-3' |
37℃ |
80℃, 20 min |
Dcm;EcoBI |
100 |
100 |
100 |
100 |
|
15032ES70 |
TthHB8I |
20 |
200T |
5'-T↓CGA-3' |
65℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
Dam |
100 |
100 |
100 |
100 |
|
15080ES25 |
NmuCI, TseFI |
10 |
25 T |
5'-↓GTSAC-3' |
65℃ |
不可热失活,请使用酚氯仿抽提或柱纯化 |
EcoBI |
100 |
12.5 |
100 |
25 |
|
15033ES76 |
无 |
20 |
500T |
5'-T↓CTAGA-3' |
37℃ |
80℃, 20 min |
Dam |
100 |
50 |
100 |
100 |
|
15060ES56 |
XcmI、Xcm I、Xcm i、Xcm1、Xcm 1、XcmⅠ、Xcm Ⅰ、Xcm |
5 |
60 T |
5'-CCA(N)5↓(N)4TGG-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
50 |
100 |
25~50 |
|
15034ES76 |
PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI |
20 |
500T |
5'-C↓TCGAG-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
|
15221ES |
BbvCI |
无 |
2 |
50 U |
5'-CC↓TCAGC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
100 |
100 |
100 |
15211ES |
PspLI, Pfl23II |
10 |
300 U |
5'-C↓GTACG-3' |
55℃ |
80℃, 20 min |
CpG |
专用缓冲液,不兼容其他 |
||||
15203ES |
无 |
10 |
1000 U |
5'-CGTCTC(N)1↓-3' |
55℃ |
80℃, 20 min |
CpG;EcoBI |
专用缓冲液,不兼容其他 |
||||
15202ES |
无 |
10 |
500U 2500U 10000 U |
5'-GCTCTTC(N)↓-3' |
50℃ |
80℃, 20 min |
无影响 |
专用缓冲液,不兼容其他 |
||||
15214ES |
Bse3DI, BseMI |
10 |
250 U |
5'-GCAATGNN↓-3' |
37℃ |
80℃, 20 min |
EcoBI |
专用缓冲液,不兼容其他 |
||||
15213ES |
无 |
10 |
500 U |
5'-CCA(N)5↓NTGG-3' |
37℃ |
80℃, 20 min |
Dcm |
25 |
100 |
25 |
100 |
|
15218ES |
无 |
5 |
250 U |
5'-TAGGGATAA↓CAGGGTAAT-3' |
37℃ |
80℃, 20 min |
无影响 |
100 |
未测试 |
未测试 |
未测试 |
|
15223ES |
Nb.BbvCI |
无 |
10 |
300 U |
5'-CCTCA GC-3' |
37℃ |
80℃, 20 min |
EcoBI |
100 |
未测试 |
未测试 |
未测试 |
15216ES |
无 |
10 |
2000 U |
5'-GCAATGNN-3' |
65℃ |
80℃, 20 min |
无影响 |
100 |
未测试 |
未测试 |
未测试 |
|
15210ES |
无 |
10 |
2000 U |
5'-GCTCTTCN-3' |
50℃ |
80℃, 20 min |
无影响 |
专用缓冲液,不兼容其他 |
||||
15217ES |
无 |
10 |
2000 U |
5'-GAGTCNNNN↓N-3' |
55℃ |
80℃, 20 min |
无影响 |
专用缓冲液,不兼容其他 |
||||
15220ES |
无 |
10 |
200 U |
5'-A↓CATGT-3' |
37℃ |
80℃, 20 min |
EcoKI;EcoBI |
50 |
<12.5% |
50 |
<12.5% |
|
15215ES |
Bse3DI, BseMI |
5 |
250 U |
5'-m5CNNG(N)9↓-3' |
37℃ |
80℃, 20 min |
Dcm;CpG |
专用缓冲液,不兼容其他 |
||||
15219ES |
无 |
10 |
500 U |
5'-CR↓CCGGYG-3' |
37℃ |
80℃, 20 min |
CpG;EcoKI;EcoBI |
50 |
<12.5% |
50 |
<12.5% |
|
15212ES |
KasI, DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI |
10 |
250 U |
5'-G↓GCGCC-3' |
37℃ |
80℃, 20 min |
CpG |
100 |
<12.5 |
100 |
<25 |
|
15222ES |
XmnI |
无 |
10 |
500 U |
5'-GAANN↓NNTTC-3' |
37℃ |
80℃, 20 min |
CpG;EcoBI |
100 |
100 |
100 |
100 |