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常用限制性内切酶选择指南(含酶切位点、同裂酶、甲基化)

 

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产品货号 产品名称 同裂酶 酶活
(U/uL)
规格
(T)
识别序列 反应温度
(ºC)
热失活方式 甲基化影响
15001ES50 FuniCut® AscI PalAI, SgsI 10 50T 5'-GG↓CGCGCC-3'
3'-CCGCGC↑GG-5'
37℃ 80℃, 20 min CpG
15002ES25 FuniCut® AvrII AspA2I, BlnI, XmaJI 5 25T 5'-C↓CTAGG-3'
3'-GGATC↑C-5'
37℃ 80℃, 20 min 无影响
15003ES76 FuniCut® BamHI 20 500T 5'-G↓GATCC-3'
3'-CCTAG↑G-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 无影响
15004ES62 FuniCut® BclI BsiQI, FbaI, Ksp22I 20 125T 5'-T↓GATCA-3'
3'-ACTAG↑T-5'
37℃ 80℃, 20 min Dam;EcoBI
15005ES50 FuniCut® BsaI 20 50T 5'-GGTCTC(N)1↓-3'
3'-CCAGAG(N)
5↑-5'
37℃ 80℃, 20 min Dcm;CpG
15006ES60 FuniCut® BstEII BstPI,Eco91I,EcoO65I,PspEI 20 100T 5'-G↓GTNACC-3'
3'-CCANTG↑G-5'
37℃ 80℃, 20 min 无影响
15007ES50 FuniCut® ClaI  BspDI,BanIII,Bsa29I,BseCI,BshVI,BsiXI,Bsp106I,BspXI,Bsu15I,BsuTUI,ZhoI 10 50T 5'-AT↓CGAT-3'
3'-TAGC↑TA-5
37℃ 80℃, 20 min Dam;CpG
15008ES50 FuniCut® DpnII BfuCI,MboI,Sau3AI,BscFI,Bsp143I,BssMI,BstENII,BstMBI,Kzo9I,NdeII 50 50T 5'-↓GATC-3'
3'-CTAG↑-5'
37℃ 80℃, 20 min Dam;EcoBI
15009ES25 FuniCut® EagI BseX3I, BstZI, EclXI, Eco52I, XmaIII 20 25T 5'-C↓GGCCG-3'
3'-GCCGG↑C-5'
37℃ 80℃, 20 min CpG
15010ES78 FuniCut® EcoRI FunII 20 600T 5'-G↓AATTC-3'
3'-CTTAA↑G-5'
37℃ 80℃, 20 min CpG
15011ES70 FuniCut® EcoRV Eco32I 20 200T 5'-GAT↓ATC-3'
3'-CTA↑TAG-5'
37℃ 80℃, 20 min CpG
15012ES76 FuniCut® HindIII 100 500T 5'-A↓AGCTT-3'
3'-TTCGA↑A-5'
37℃ 80℃, 20 min EcoBI
15013ES50 FuniCut® HpaI BstHPI, KspAI 5 50T 5'-GTT↓AAC-3'
3'-CAA↑TTG-5'
37℃ 80℃, 20 min CpG;EcoK
15015ES70 FuniCut® KpnI 100 200T 5'-GGTAC↓C-3'
3'-C↑CATGG-5'
37℃ 80℃, 20 min 无影响
15016ES60 FuniCut® MluI 20 100T 5'-A↓CGCGT-3'
3'-TGCGC↑A-5'
37℃ 80℃, 20 min CpG
15018ES30 FuniCut® NcoI Bsp19I 20 30T 5'-C↓CATGG-3'
3'-GGTAC↑C-5'
37℃ 80℃, 20 min 无影响
15019ES70 FuniCut® NdeI FauNDI 20 200T 5'-CA↓TATG-3'
3'-GTAT↑AC-5'
37℃ 80℃, 20 min 无影响
15020ES30 FuniCut® NheI AsuNHI 20 30T 5'-G↓CTAGC-3'
3'-CGATC↑G-5
37℃ 80℃, 20 min CpG
15021ES50 FuniCut® NotI CciNI 20 50T 5'-GC↓GGCCGC-3'
3'-CGCCGG↑CG-5'
37℃ 80℃, 20 min CpG
15022ES76 FuniCut® PstI BspMAI 20 500T 5'-CTGCA↓G-3'
3'-G↑ACGTC-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 无影响
15023ES60 FuniCut® SacI Psp124BI, SstI 20 100T 5'-GAGCT↓C-3'
3'-C↑TCGAG-5'
37℃ 80℃, 20 min 无影响
15024ES70 FuniCut® SalI 20 200T 5'-G↓TCGAC-3'
3'-CAGCT↑G-5'
37℃ 80℃, 20 min CpG
15025ES25 FuniCut® SbfI SdaI, Sse8387I 20 25T 5'-CCTGCA↓GG-3'
3'-GG↑ACGTCC-5'
37℃ 80℃, 20 min 无影响
15027ES60 FuniCut® SmaI 20 100T 5'-CCC↓GGG-3'
3'-GGG↑CCC-5'
25℃ 80℃, 20 min CpG
15028ES50 FuniCut® Spel AhlI, BcuI 20 50T 5'-A↓CTAGT-3'
3'-TGATC↑A-5'
37℃ 80℃, 20 min EcoBI;EcoK
15029ES50 FuniCut® SphI BbuI, PaeI, SpaHI 20 50T 5'-GCATG↓C-3'
3'-C↑GTACG-5'
37℃ 80℃, 20 min EcoBI
15030ES56 FuniCut® SspI 20 60T 5'-AAT↓ATT-3'
3'-TTA↑TAA-5'
37℃ 80℃, 20 min 无影响
15031ES60 FuniCut® StuI AatI, Eco147I, PceI, SseBI 10 100T 5'-AGG↓CCT-3'
3'-TCC↑GGA-5'
37℃ 80℃, 20 min Dcm;EcoBI
15032ES70 FuniCut® TaqI TthHB8I 20 200T 5'-T↓CGA-3'
3'-AGC↑T-5'
65℃ 不可热失活,请使用酚氯仿抽提或柱纯化 Dam
15033ES76 FuniCut® XbaI 20 500T 5'-T↓CTAGA-3'
3'-AGATC↑T-5'
37℃ 80℃, 20 min Dam
15034ES76 FuniCut® XhoI PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI 20 500T 5'-C↓TCGAG-3'
3'-GAGCT↑C-5'
37℃ 80℃, 20 min CpG
15036ES50 FuniCut® FspI Acc16I, Avill, MstI, NsbI 10 50T 5'-TGC↓GCA-3'
3'-ACG↑CGT-5'
37℃ 80℃, 20 min CpG
15038ES76 FuniCut® HinfI 50 500T 5'-G↓ANTC-3'
3'-CTNA↑G-5
37℃ 80℃, 20 min CpG;EcoBI
15039ES70 FuniCut® ApaLI 10 200T 5'-G↓TGCAC-3'
3'-CACGT↑G-5'
37℃ 80℃, 20 min CpG
15040ES50 FuniCut® NruI 20 50T 5'-TCG↓CGA-3'
3'-AGC↑GCT-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 CpG;Dam
15041ES25 FuniCut® PacI 10 25T 5'-TTAAT↓TAA-3'
3'-AAT↑TAATT-5'
37℃ 80℃, 20 min EcoK
15042ES70 FuniCut® PvuII 20 200T 5'-CAG↓CTG-3'
3'-GTC↑GAC-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 EcoBI
15043ES50 FuniCut®  SacII Cfr42I,KspI,Sfr303I,SgrBI 20 50T 5'-CCGC↓GG-3'
3'-GG↑CGCC-5'
37℃ 80℃, 20 min CpG
15044ES25 FuniCut®  NsiI EcoT22I, Mph1103I, Zsp2I 20 25T 5'-ATGCA↓T-3'
3'-T↑ACGTA-5'
37℃ 80℃, 20 min EcoBI
15048ES30 FuniCut®  Esp3I (BsmBI) BsmBI, BstGZ53I, Esp16I, Esp23I 10 30T 5'-CGTCTC(N)1↓-3'
3'-GCAGAG(N)5↑-5'
37℃ 80℃, 20 min CpG;EcoBI
15049ES60 FuniCut®  BglII 50 100T 5'-A↓GATCT-3'
3'-TCTAG↑A-5'
37℃ 80℃, 20 min EcoBI
15050ES60 FuniCut®  BstBI AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI 20 100T 5'-TT↓CGAA-3'
3'-AAGC↑TT-5'
37℃ 80℃, 20 min CpG
15051ES50 FuniCut®  MnlI 5 50T 5'-CCTCN7↓-3'
3'-GGAGN6↑-5'
37℃ 80℃, 20 min EcoBI
15052ES50 FuniCut®  DpnI 20 50T 5'-GAm6↓TC-3'
3'-CT↑Am
6G-5'
37℃ 80℃, 20 min CpG
15056ES25 FuniCut®  AgeI AsiGI, CspAI, BshTI, PinAI 20 25 T 5'-A↓CCGGT-3'
3'-TGGCC↑A-5'
37℃ 80℃, 20 min CpG
15057ES60 FuniCut®  SfiI 20 100 T 5'-GGCCN4↓NGGCC-3'
3'
-CCGGN↑N4CCGG-5'
50℃ 不可热失活,请使用酚氯仿抽提或柱纯化 Dcm;CpG
15058ES70 FuniCut®  PspGI EcoRII, MvaI, BstNI, BciT130I, BseBI, AjnI, Psp6I, Bst2UI 10 200 T 5'-↑CCWGG-3'
3'
-GGWCC↑-5'
75℃ 不可热失活,请使用酚氯仿抽提或柱纯化 Dcm
15059ES60 FuniCut®  DraI 5 100 T 5'-TTT↓AAA-3'
3'-AAA↑TTT-5'
37℃ 80℃, 20 min EcoK
15060ES56 FuniCut®  XcmI XcmI、Xcm I、Xcm i、Xcm1、Xcm 1、XcmⅠ、Xcm Ⅰ、Xcm 5 60 T 5'-CCA(N)5↓(N)4TGG-3'
3'-GGT(N)4↑(N)5ACC-5'
37℃ 80℃, 20 min 无影响
15061ES30 FuniCut® BsrGI BstAUI, Bsp1407I 20 30 T 5'-T↓GTACA-3'
3'-ACATG↑T-5'
37℃ 80℃, 20 min 无影响
15062ES50 FuniCut® PmeI MssI 20 50 T 5'-GTTT↓AAAC-3'
3'-CAAA↑TTTG-5'
37℃ 80℃, 20 min CpG
15063ES70 FuniCut® HpaII MspI, HapII, BsiSI 20 200 T 5'-C↓CGG-3'
3'-GGC↑C-5'
37℃ 80℃, 20 min CpG
15064ES72 FuniCut® MspI HpaII, HapII, BsiSI 10 250 T 5'-C↓CGG-3'
3'-GGC↑C-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 无影响
15065ES50 FuniCut® AccI XmiI, FblI 10 50 T 5'-GT↓MKAC-3'
3'-CAKM↑TG-5'
37℃ 80℃, 20 min CpG
15066ES30 FuniCut® EarI Eam1104I, Bst6I 20 30 T 5'-CTCTTC(N)₁↓-3'
3'- GAGAAG(N)
₄↑-5'
37℃ 80℃, 20 min CpG
15067ES75 FuniCut® HaeIII BsuRI, BshFI, BspANI, BsnI 10 300 T 5'-GG↓CC-3'
3'-CC↑GG-5'
37℃ 80℃, 20 min 无影响
15068ES25 FuniCut® MunI (MfeI) MfeI 20 25 T 5'-C↓AATTG-3'
3'-GTTAA↑C-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化 无影响
15069ES30 FuniCut® PvuI Ple19I 20 30 T 5'-CG AT↓CG-3'
3'-GC↑TAGC-5'
37℃ 80℃, 20 min CpG
15070ES25 FuniCut® BbsI BstV2I, BpiI 20 25 T 5'-GAAGAC(N)₂↓-3'
3'-CTTCTG(N)
₆↑
37℃ 80℃, 20 min 无影响
15071ES50 FuniCut® DdeI BstDEI, HpyF3I 10 50 T 5'-C↓TNAG-3'
3'-GANT↑C-5'
37℃ 80℃, 20 min EcoBI 
15072ES60 FuniCut® HhaI HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI 20 100 T 5'-GCG↓C-3'
3'-C↑GCG-5'
37℃ 80℃, 20 min CpG
15073ES60 FuniCut® HinP1I HhaI, CfoI, AspLEI, Hin6I, HspAI, BstHHI 10 100 T 5'-G↓CGC-3'
3'-CGC↑G-5'
37℃ 80℃, 20 min CpG
15074ES30 FuniCut® DraIII AdeI 20 30 T 5'-CAC(N)3↓GTG-3'
3'-GTG↑(N)3CAC-5'
37℃ 不可热失活,请使用酚氯仿抽提或柱纯化  CpG;EcoKI;EcoBI
15075ES70 FuniCut® BglI 10 200 T 5'-GCC(N)4↓NGGC-3'
3'-CGGN↑(N)4CCG-5'
37℃ 80℃, 20 min CpG
15202ES BspQI(10 U/μL) 10

500 U

2500 U

10000 U

5'-GCTCTTC(N)↓-3'
3'
-CGAGAAG(N)4-5'
50℃ 80℃, 20 min 无影响
15203ES BsmBI(10 U/μL) 10 1000 U 5'-CGTCTC(N)1↓-3'
3'-GCAGAG(N)5↑-5'
55℃ 80℃, 20 min CpG;EcoBI
15210ES Nt.BspQI(10 U/μL) 10 2000 U 5'-GCTCTTCN-3'
3'-CGAGAAGN-5'
50℃ 80℃, 20 min 无影响
15211ES BsiWI(10 U/μL) PspLI, Pfl23II 10 300 U 5'-C↓GTACG-3'
3'-GCATG↑C-5'
55℃ 80℃, 20 min CpG

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