产品说明书
FAQ
COA
已发表文献
Programmed Death-1 receptor, also known as PD-1 and CD279, is a receptor expressed on T cells responsible for modulating T cell activation. Like CTLA 4, PD-1 is classified as an immune checkpoint inhibitory receptor. Mature human PD-1 consists of an extracellular region (ECD) with one immunoglobulin-like V type domain, a transmembrane domain, and a cytoplasmic region. The mature ECD of human PD-1 shares 61% amino acid sequence identity with mouse PD-1 ECD.
产品性质
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别名（Synonyms） |
PDCD1, PD1, CD279, SLEB2 |
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Uniprot No. |
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表达区间及表达系统（Source） |
HEK293 Cells-derived Human PD-1 / PDCD1 Protein, Leu25-Gln167 with His tag at the C-terminal |
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分子量(Molecular Weight) |
Approximately 17.0 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Human PD-1, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 45.0 ng/mL determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1.避免反复冻融。
2.为了您的安全和健康，请穿实验服并戴一次性手套操作。
3.本产品仅作科研用途！
产品数据
HB230811
