产品说明书
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产品信息
|
产品名称 |
产品编号 |
规格 |
|
Recombinant Human LIGHT / TNFSF14 |
93747ES25 |
25 μg |
|
93747ES60 |
100 μg |
|
|
93747ES76 |
500 μg |
产品描述
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.
产品性质
|
别名（Synonyms） |
CD258, TNFSF14, HVEM-L, LIGHT, LTg, TR2 |
|
Uniprot No. |
|
|
表达区间及表达系统（Source） |
HEK293 Cells-derived Human LIGHT / TNFSF14 Protein, Asp74-Val240 with hFc tag at the N-terminal |
|
分子量(Molecular Weight) |
Approximately 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
1.ELISA Data: Immobilized Human LIGHT, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12 μg/mL determined by ELISA. 2.ELISA Data: Immobilized Human LIGHT, His Tag at 0.5 μg/ml (100 μL/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 of 22.9 ng/mL determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
- 避免反复冻融。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
- 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
SEC-HPLC
ELISA Data
ELISA Data
