Recombinant PE-Labeled Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein,His-Avi Tag

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产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

表达区间及表达系统(Source)

PE-Labeled Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human KRAS G12V (HLA-A*03:01) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide.[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK]

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

活性(Activity)

ELISA Data: Immobilized HLA-A*11:01&B2M&KRAS G12D TCR at 1μg/ml (100μl/well) on the plate. Dose response curve for PE Labeled Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 0.11μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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