胰岛素是胰腺中胰岛β细胞分泌的一种双链形式(α,β)的多肽激素,由51个氨基酸组成,分子量约5800 Da。两条链间通过二对二硫键连接,而α链自身还含有一对二硫键。胰岛素是目前已知的机体内能降低血糖的蛋白激素,不仅负责调控细胞对葡萄糖、氨基酸和脂肪酸的摄入,利用和储存,而且抑制糖原,蛋白质和脂肪的降解发生。
不同物种胰岛素功能大体相同,只是氨基酸组成上的差异。人胰岛素结构见下:
CAS号(CAS NO.) |
11070-73-8 |
分子式(Molecular Formula) |
C254H377N65O75S6 |
分子量(Molecular Weight) |
5733.5 |
外观(Appearance) |
白色至类白色粉末 |
活性(potency)(HPLC) |
≥27 USP units/mg |
锌含量(Zinc Amount ) |
~0.5%(若实验要求去除锌离子,可将胰岛素溶解在稀醋酸内,加入过量EDTA螯合锌离子,然后等电点沉淀纯化胰岛素) |
溶解性(Solubility) |
不溶于水,溶于稀醋酸或稀盐酸(pH 2-3) |
等电点(pI) |
pl = 5.3 (天然蛋白) |
沉降系数(S20,w) (Sedimentation Coefficient) |
1.95 x 10-13 |
扩散系数(D20,w) (Diffusion Coefficient) |
7.30×10-7 |
EM(278nm) |
6080(33 mM phosphate,pH 7.0) |
E1%(278nm) |
10.6(33 mM phosphate,pH 7.0) |
序列(Sequence) |
A-Chain: G-I-V-E-Q-C-C-A-S-V-C-S-L-Y-Q-L-E-N-Y-C-N B-Chain: F-V-N-Q-H-L-C-G-S-H-L-V-E-A-L-Y-L-V-C-G-E-R-G-F-F-Y-T-P-K-A |
Q:我想问下我们购买的贵司的Insulin,它的溶解方法只能是用盐酸溶解吗?可不可以用其他溶剂?用盐酸溶解的 insulin 适用于临床吗?
A:1.也可用于稀醋酸(pH 2-3)配制母液。2.只能用于科研实验,不能用于临床。
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