c-Kit /CD117, is a receptor tyrosine kinase. Binding of SCF R to SCF, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages. Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility. Within the ECD, human SCF R shares 73% and 76% aa sequence identity with mouse and rat SCF R, respectively.

 

产品性质

Synonyms	SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT
Uniprot No.	P10721.2
Source	Recombinant Human CD117 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln26-Thr516.
Molecular Weight	Approximately 56.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
Purity	> 95% as determined by SDS-PAGE and HPLC.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

The products are shipped with ice pack and can be stored at -20℃ for 1 year.

2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3-6 months, -20 to -80°C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

 

注意事项

1.For your safety and health, please wear lab coats and disposable gloves for operation.

2.For research use only!