产品说明书
FAQ
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已发表文献
产品描述
Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex. This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation.
产品性质
别名 |
UNQ3115; PRO10196; FGF-21; FGF21 |
Uniprot No. |
Q9NSA1.1 |
表达区间及表达系统 |
Recombinant Human FGF21 Protein is expressed from HEK293 Cells with mFc tag and Avi tag at the N-terminal. It contains His29-Ser209. |
分子量 |
Approximately 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
活性 |
ELISA Data：Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. SPR Data：Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200). |
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220811
