您好,欢迎来到YEASEN!
400-6111-883
400-6111-883
新闻中心
首页 > 新闻中心 > 产品速递
干货!AOM/DSS结直肠癌模型

AOM/DSS模型是目前使用最广泛的UC化学诱导模型,临床症状学、形态学及病理学观察证明,该模型与人类溃疡性结肠炎癌变过程极为相似。AOM引起鸟嘌呤O6甲基化是诱导基因突变损伤最主要的原因,基因突变使细胞过度增殖,形成特异性的大肠肿瘤。DSS的致炎机制尚未阐明,可能与DSS的负电荷影响 DNA 合成、抑制上皮细胞增生、破坏肠黏膜屏障、导致巨噬细胞功能障碍及肠道菌群失调有关。


 1.png

1.来源pixabay

 



作用机制


偶氮甲烷(AOM)能诱导DNA产生O6-甲基鸟嘌加合物,导致G→A转化。



实验动物


 BALB/c小鼠(7周龄,雄性)



实验材料


DSS(Yeasen#60316ES,MW36000-50000)、AOM



实验前准备


DSS配制:使用无菌水配制浓度1-3% DSS溶液,储存在4℃备用。

AOM配制:用无菌水溶解AOM配制10 mg/mL的储存液,分装后放到-20℃保存,避免反复冻融。使用之前,融化分装母液并用无菌生理盐水1:10倍稀释。

 



实验步骤


1.第1天称重并标记小鼠,实验组小鼠腹腔注射偶氮甲烷 AOM(10 mg/kg);
2.用常规饮用水喂食一周;
3.用2.5%浓度DSS替换饮用水喂食一周;
4.用常规饮用水喂食2周;
5.步骤3和步骤4重复3次。 

2.png 

2.AOM/DSS结直肠癌造模步骤



结果分析


 


3.png

3.(A)AOM/DSS 诱导的结直肠癌示意图, (B)小鼠体重、(C)腹泻评分、(D)出血评分和(E) 代表性全结肠和结肠癌数的变化。

 

4.png 

4.结肠切片HE染色结果

 



参考文献


[1] Jia-Rong Huang, Sheng-Te Wang,Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.11:586885.(IF=5.988,DSS yeasen 60316ES)

 

翌圣DSS产品优势


 

翌圣生物提供高品质DSS(Cat.NO:60316ES,MW:36000~50000),具有以下优势:

1. 高纯度(>98%),硫含量17-19%,游离硫<0.2%,大量数据文献支持,广泛应用于UC模型的构建。

2. 多种属动物中均可造模:小鼠、大鼠、斑马鱼、猪、果蝇;

3. 症状表现与人UC极度相似:可用于研究急、慢性结肠炎的发生发展机制,也可用于药物的药效研究。

4. 简单易行,成模率高,重复性强

5. 联合氧化偶氮甲烷 (azoxymethane, AOM)用药,可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型,成功模拟IBD诱发CAC的过程。

5.png 

 

建模效率与进口产品一致,产品价格低至进口价格的1/3,大量现货库存。

 

产品订购


 

产品名称

货号

规格

价格(元)

促销价(元)

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES25

25 g

1255

955

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES60

100 g

3855

2655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES76

500 g

16855

9655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES80

1 kg

32855

18655

点击产品名称查看详情


产品已发表文章(部分)

向下滑动查看


2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

 

2021

[1] Li Zhao, Fei Wang , Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].Chemical Engineering Journal.424(2021)130464. IF=16.744

[2] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

 

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

 

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943





 

 



往期精彩


1.不同分子量葡聚糖硫酸钠选购指南:高品质DSS葡聚糖硫酸钠盐

2.小鼠造模方法及具体步骤:DSS结肠炎造模解决方案

3.DSS结肠炎造模整体解决方案:Dextran Sulfate Sodium Salt(DSS) 溃疡性结肠炎模型的建立 (yeasen.com)