攻无不“克”的无缝克隆为何备受青睐？
导读
传统酶切克隆
无缝克隆
1. 不受片段、载体序列的限制，可任意组装
2. 可同时无缝地拼接多个片段
3. 操作简便，时间上大大节省
1. 线性化载体制备
2. PCR获取目的插入片段
3. 重组反应
1. Jiang, Y., et al., Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi[J]. Science, 2017. (IF 37.205)
2. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019. (IF 31.398)
3. Xing, Y.H., et al., SLERT Regulates DDX21 Rings Associated with Pol I Transcription[J]. Cell, 2017. 169(4): p. 664-678.e16. (IF 31.398)
4. Wang Y, Hu SB, et al. Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria[J]. Nat Cell Biol. 2018 Oct;20(10):1145-1158. (IF 20.46)
5. Li X., et al., Coordinated circRNA Biogenesis and Function with NF90/NF110 in Viral Infection[J]. Mol Cell. 2017 Jul 20;67(2):214-227.e7. (IF 14.548)
6. Yao R W, Xu G, Wang Y, et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. Molecular cell, 2019. (IF 14.548)
7. Wu P, Zhang T, Liu B, et al. Mechano-regulation of peptide-MHC class I conformations determines TCR antigen recognition[J]. Molecular cell, 2019, 73(5): 1015-1027. e7. (IF 14.548)
8. Qiao HH, Wang F, et al. An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila[J]. Nat Commun. 2018 Oct 8;9(1):4160. (IF 12.353)
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