Molefuture Biotechnology, a subsidiary of Yeasen Biotechnology (Shanghai) Co., Ltd., specializes in tailored enzyme modification solutions driven by cutting-edge enzyme evolution technologies.  These solutions cater to diverse application needs in areas such as in vitro diagnostics, biopharmaceuticals, synthetic biology, medical aesthetics, pharmaceutical intermediates, and beyond.

ZymeEditor enzyme evolution platform

The ZymeEditor platform seamlessly integrates directed evolution and rational design techniques. It autonomously incorporates cutting-edge enzyme evolution technologies, including Fluorescence-Activated Droplet Sorting (FADS), alongside traditional methods like Microtiter Plate Screening (MTPS) and forfront Computer-Aided Rational Design (CARD). Through cross-screening utilizing these technologies, ZymeEditor significantly enhances the efficacy of enzyme evolution, leading to the optimization of various enzyme properties such as activity, stability, selectivity, and more.

To address the growing need for large-scale production of top-quality enzymes, Molefuture, supported by its parent company Yeasen, has erected two GMP-compliant production facilities spanning nearly 10,000 square meters in Wuhan, China. Additionally, we've installed two state-of- the-art, fully automated 1500-liter fermentation systems and assembled a dedicated team of experts specializing in enzyme production and quality assurance. These resources collectively ensure the successful scale-up production of  high-caliber enzymes.  

Case Studies

1. Reducing T7 RNA polymerase dsRNA by-products

1) ELISA for dsRNA content shows that mutants M1-5 have lower dsRNA levels, with M5 reducing by 98% compared to WT and 78% compared to a competitor (Fig 3- a).

2) Dot blot analysis confirms that mutants M1-5 have reduced dsRNA content (Fig 3-b)

2. Enhancing stability and  reducing adapter-adapter ligation of T4 DNA Ligase

1) By protein engineering, the stability of T4 DNA ligase mutants increased significantly without sacrificing their activity. M6 could keep its activity 100% under 45 degree(Fig 2).  

2) The Qseq tests showed that the adapter-adapter ligation ratio of the mutants decreased from 10% of the wild type to about 3%, and even lower to 1% of M1 and M4(Fig 3).

3) After incubation at 42℃ for 1h, the DNA yields of mutants increased dramatically in Preparation of DNA Libraries(Fig 4).