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DSS溃疡性结肠炎造模-猪模型

背景概述


肠道通透性增加和相关体重减轻是DSS结肠炎动物模型的常⻅特征。D-甘露醇是⼀种惰性小碳水化合物分⼦,可沿整个隐窝-轴吸收,⽤于评估体内肠道通透性。

1663578086242310.png 

图源百度


实验动物

4-5天大约克郡小猪

实验材料

DSS(yeasen DSS 60316ES,MW36000-50000)

实验步骤

1. 以商业代乳粉配⽅饮⾷,每天喂食三次,直到实验结束

2. 将动物分为阳性对照组(Pos),阴性对照组(Neg),实验组(Trp);

3. 阳性对照组(Pos):灌注DSS;阴性对照组(Neg):灌注生理盐水;实验组(Trp):灌注DSS+治疗药物

4. 每只小猪每日摄入量1.25g DSS/kg,灌注5天

实验结果



1663578193907808.png 

1. 肠道通透性分析

DSS诱导致小猪体内D-甘露醇浓度升高,肠道屏障被破坏。

1663578216471123.png 

2. HE病理染色切片(A为Neg组;B为Pos组)


DSS处理的Pos组
隐窝结构扭曲、炎症细胞浸润到粘膜和粘膜下层、隐窝脓肿和隐窝炎

1663578246943424.png 

3. 炎症因子检测

DSS处理的Pos组中两种细胞因⼦的浓度均升高[1]

 

翌圣DSS产品优势

翌圣提供高品质DSS(Cat.NO:60316ES,MW:36000~50000)具有以下优势:

1、高纯度98%),硫含量17-19%游离硫<0.2%,大量数据文献支持广泛应用于UC模型的构建。

2、多种属动物中均可造模:小鼠、大鼠、斑马鱼、猪、果蝇;

3、症状表现与人UC极度相似:可用于研究急、慢性结肠炎的发生发展机制,也可用于药物的药效研究。

4、简单易行,成模率高,重复性强

5、联合氧化偶氮甲烷 (azoxymethane, AOM)用药,可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型,成功模拟IBD诱发CAC的过程。

1663578309396476.png

建模效率与进口产品一致,产品价格低至进口价格的1/3,大量现货库存。


产品订购


产品名称

货号

规格

价格(元)

促销价(元)

Dextran Sulfate Sodium Salt(DSS)

结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES25

25 g

1255.00

955.00

Dextran Sulfate Sodium Salt(DSS)

结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES60

100 g

3855.00

2655.00

Dextran Sulfate Sodium Salt(DSS)

结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES76

500 g

16855.00

9655.00

Dextran Sulfate Sodium Salt(DSS)

结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES80

1 kg

32855.00

18655.00

 


产品已发表文章(部分)

向下滑动查看

2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744

[2] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245


往期精彩

1. 不同分子量葡聚糖硫酸钠选购指南:高品质DSS葡聚糖硫酸钠盐

2. 小鼠造模方法及具体步骤:Dextran Sulfate Sodium Salt(DSS)结肠炎造模解决方案 (yeasen.com)

3. DSS结肠炎造模整体解决方案:Dextran Sulfate Sodium Salt(DSS) 溃疡性结肠炎模型的建立 (yeasen.com)


DSS(60316ES)参与发SCI赢奖励活动,最高可得2000元礼品

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参考文献

[1] Connie J. Kim, et al. Journal of Nutritional Biochemistry 21 (2010) 468–475.