Abstract

Conventional gelatin-based hydrogel wound dressings suffer from weak mechanical properties, poor temperature stability, and slow wound healing, limiting their practical application. Herein, a novel multifunctional gelatin-based eutectogel dressing (PGEWD) was developed via a multicross-linked network constructed from porcine skin gelatin (PG), ε-polylysine (EPL), waterborne polyurethane (WPU), and deep eutectic solvent (DES, choline chloride-glycerol). DES induced PG molecular chain rearrangement to form a dense triple-helix structure. With the optimal formulation of 10% EPL, 0.3% WPU, and 10 min DES immersion, the PGE0.1W0.3D10 composite exhibited high tensile strength (290 kPa), intrinsic conductivity (0.798 mS/cm), wide thermal tolerance (−20 to 60 °C), and ∼100% antibacterial activity. Combined with electrical stimulation (ES), it accelerated wound healing with ∼94.47% closure rate in 14 days. This study provides a versatile strategy for designing multifunctional gelatin-based wound dressings with significant potential in wound regeneration.