斑马鱼作为肠道损伤模型的应用已得到充分证实，葡聚糖硫酸钠（Dextran Sulfate Sodium Salt，DSS）诱导的斑马鱼IBD模型模拟了哺乳动物IBD表型的不同特征，包括肠道中性粒细胞炎症、过多粘液生成、肠淋巴管生成增加以及前炎性细胞因子上调。

图片来源：包图网

实验动物

3dpf（days post fertilization) 斑马鱼

实验材料

DSS（Yeasen 60316ES，MW:36000~50000）

实验步骤

1. 将斑马鱼胚胎培养在含甲基蓝的E3胚胎培养基中，28.5 ℃，培养至1 dpf；

2. 1dpf 后使用不含亚甲基蓝的E3胚胎培养基，培养至3 dpf；

3. 用E3培养基配制浓度为10% DSS的储存液;

4. 用培养基稀释DSS至最大非致死剂量（DSS浓度参考：0.5%）；

5. 用0.5%的DSS处理斑马鱼，从3 dpf处理到6 dpf，观察指标^[1]。

结果分析

造模成功指标：中性粒细胞数量增多、酸性粘液蛋白增多、炎性因子表达量上升。

1.嗜中性粒细胞数量：中性粒细胞数量增多，并且向肠和上皮中移动

2.酸性粘液蛋白：石蜡病理切片结合AB-PAS染色法验证，DSS浸泡后斑马鱼肠道中酸性粘液蛋白增多

3.炎性因子表达量：DSS处理后斑马鱼肠道中炎性因子表达量上升^[1,2]

客户案例

图1.Yeasen DSS（Cat#60316ES）斑马鱼造模实验结果（Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557）

DSS处理组相较于对照组，酸性粘蛋白染色和粘液分泌增加，斑马鱼外周血中的嗜中性粒细胞数量增多并且向肠和上皮中移动，炎性因子表达量显著上调，肠道出现炎症反应。

翌圣DSS产品优势

翌圣提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：

1.高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建；

2.多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；

3.症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究；

4.简单易行，成模率高，重复性强；

5.联合氧化偶氮甲烷 (azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。

建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。

产品订购

产品名称	货号	规格
ColitCare^® Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES25	25 g

ColitCare^® Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES60	100 g

ColitCare^® Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES76	500 g

ColitCare^® Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES80	1 kg

点击产品名称查看详情

其他产品推荐

货号	产品名称	规格	应用场景
18820ES	MolPure® Stool DNA Kit 粪便DNA提取试剂盒	5 T/50 T/200 T	能高效获得优质的粪便DNA，有利于后续对肠道微生物的研究。
18528ES	MolPure® Mag48 Soil/Stool DNA Kit FA 磁珠法48孔土壤 /粪便DNA提取试剂盒FA（预封装）	48 T	磁珠法提取样本基因组DNA。
18526ES	MolPure®Mag Soil/Stool DNA Kit 磁珠法土壤/粪便DNA提取试剂盒	20 T/50 T/200 T	磁珠法提取样本基因组DNA。
60524ES	Hematoxylin and Eosin Staining Kit 苏木素伊红(H&E)染色试剂盒	2×100 mL	用于免疫组化中组织切片，血涂片，骨髓切片的染色。
60403ES	Urine Fecal Occult Blood Test Kit 尿粪隐血检测试剂盒(联苯胺法)	100T	对慢性消化道出血，如：消化性溃疡等的研究筛选均具有重要价值。
60534ES	AB-PAS染色液	6×50 mL/6×100 mL	可鉴别同一组织中的中性黏蛋白和酸性黏蛋白。
60751ES	Azoxymethane 偶氮甲烷(AOM) \|CAS 25843-45-2	1 mg/5 mg/10 mg（液体：135 mM in Water）	与葡聚糖硫酸钠盐DSS 一起用于结直肠癌的小鼠模型创建。

产品已发表文章(部分)

向下滑动查看

2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943

相关专题

1.不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐

2.DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS）溃疡性结肠炎模型的建立 (yeasen.com)

参考文献：[1] Oehlers SH, et al. Dis Model Mech. 2012 Jul;5(4):457-67.[2] Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557.