Recombinant Human VEGF165 Protein (HEK293)

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产品说明书

FAQ

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已发表文献

 

VEGF 165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF. 

 

性能参数

Synonyms

VEGF-165VEGF; VEGFA; MVCD1;  VPF; RP1-261G23.1;

Uniprot No.

P15692-4

Source

Recombinant Human VEGF165 Protein is expressed from HEK293 without tag. It contains Ala27-Arg191

Molecular Weight

The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.

Biological Activity

ED50 < 3 ng/ml, measured in a cell proliferation assay using HUVEC cells.

Purity

> 95% by SDS-PAGE and HPLC

Endotoxin

<1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20231218

400-6111-883