Recombinant Mouse FLT3 Ligand Protein,His Tag 重组小鼠fms相关受体酪氨酸激酶3配体

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产品说明书

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产品描述

 

Flt3 配体,也称为 FL,是一种α-螺旋细胞因子,可促进多种造血细胞谱系的分化。通过激活 FLT3 刺激早期造血细胞的增殖。与许多其他集落刺激因子和白细胞介素有很好的协同作用。

 

性能参数

 

蛋白别名(Synonyms)

FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine

种属(Species)

Mouse

表达系统(Source)

HEK293

表达序列(Sequence)

Gly27-Gln189

Accession

P49772-1

标签(Tag)

C-His

分子量(Molecular Weight)

The protein has a predicted MW of 19.61 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.

内毒素(Endotoxin)

< 1 EU/μg by the LAL method.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE;  > 95% as determined by HPLC

活性(Activity)

ELISA Data: Immobilized Mouse FLT3 Ligand, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 39.3ng/ml determined by ELISA.


SPR Data: Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观(Appearance)

冻干粉

 

储存条件

 

-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。

 

复溶方法

 

开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。

 

注意事项

 

1.为了您的安全和健康,请穿实验服并戴一次性手套操作。

2.本产品仅作科研用途

 

Ver. CN20240919

 

400-6111-883