蛋白别名（Synonyms）

R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1

种属（Species)

Mouse

表达系统（Source）

HEK293

表达序列（Sequence）

Ser21-Gln265

Accession

Q9Z132

标签（Tag)

C-His

分子量（Molecular Weight）

The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.

内毒素（Endotoxin）

< 1 EU/μg by the LAL method.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE

活性（Activity）

ELISA Data: Immobilized Mouse R spondin 1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.

Cell Based Assay: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4 - 20 ng/mL in

the presence of 5 ng/mL recombinant Human Wnt3a .

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.

外观（Appearance）

冻干粉