R-Spondin-1(Rspo-1)属于Wnt调节剂的(Rspo)家族。目前,该家族由四个结构相关的分泌配体(Rspo 1-4)组成,所有配体都含有弗林样结构域和血小板反应蛋白结构域。Rspo-1在发育中的中枢神经系统的某些区域以及肾上腺、卵巢、睾丸、甲状腺和气管中表达。Rspo可以以刺激Wnt/β-catenin信号通路的方式与Frizzled/LRP6受体复合物相互作用。随着类器官领域的扩大,R-Spondin 1被广泛用于类器官细胞培养中,作为促进3D类器官生长和存活的重要成分。
高纯度、高活性、低内毒素、高批间一致性
SDS-PAGE

Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
ELISA Data

Immobilized Mouse R spondin 1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
Cell Based Assay

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4 - 20 ng/mL inthe presence of 5 ng/mL recombinant Human Wnt3a.
-25 ~ -15℃保存,收到货之后有效期1年。
复溶后, -85 ~ -65℃保存,3个月有效期。





