利用工程化光开关RNA结合蛋白实现RNA功能与代谢的光遗传学控制
Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins
Liu Renmei, Yang Jing, Yao Jing, Zhao Zhou, He Wei, Su Ni, Zhang Zeyi, Zhang Chenxia, Zhang Zhuo, Cai Haibo, Zhu Linyong, Zhao Yuzheng, Quan Shu, Chen Xianjun, Yang Yi
Journal：NATURE BIOTECHNOLOGY
IF：68.16
DOI：10.1038/s41587-021-01112-1
PMID：34980910
Published：2022-01-03
research field：分子生物学合成生物学血液学
Abstract
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in response to blue light irradiation. When fused to various RNA effectors, LicV allows for optogenetic control of RNA localization, splicing, translation and stability in cell culture. Furthermore, LicV-assisted CRISPR–Cas systems allow for efficient and tunable photoswitchable regulation of transcription and genomic locus labeling. These data demonstrate that the photoswitchable RBP LicV can serve as a programmable scaffold for the spatiotemporal control of synthetic RNA effectors.
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