Biotinylated Recombinant Human GPC3/Glypican 3 Protein, His-Avi Tag

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产品说明书

FAQ

COA

已发表文献

 

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

 

产品性质

Synonyms

GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1

Uniprot No.

 P51654-1

Source

Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln25-His559.

Molecular Weight

The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 80-135 kDa based on Tris-Bis PAGE result.

Purity

> 90% as determined by Tris-Bis PAGE

Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

The products are shipped with ice pack and can be stored at -20 for 1 year.

2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 -6 months, -20 to -80°C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

 

注意事项

1.For your safety and health, please wear lab coats and disposable gloves for operation.

2.For research use only!

 

产品数据

HB221206

400-6111-883