Abstract

Polychlorinated biphenyls (PCBs) are a class of persistent organic pollutants distributed worldwide. Existing researches indicated that the immune system is one of the most sensitive indicators of adverse health effects caused by PCBs. We for the first time evaluated the immunotoxic effect of PCB29-pQ, an active quinone-type PCB metabolite. After PCB29-pQ exposure, the body weight of the mice was reduced, but increased the organ index of the spleen and lungs. The morphology and structure of the mouse spleen and lungs were changed, and partial types of lymphocyte subsets in the spleen were significantly reduced. The activation of caspase-3, the significant up-regulation of Bax and the decrease of Bcl-2 indicated occurrence of apoptosis. In addition, mRNA results showed that PCB29-pQ caused the imbalance of Th1/Th2 cytokines and promoted the Th1-type immune response. Taken together, the above results demonstrated that treatment with PCB29-pQ induced spleen immune dysfunction targeting the apoptosis pathway and Th1/Th2 cytokines imbalance in mice. Since the immune system plays a fundamental role in maintaining homeostasis and is strongly involved in the development of diseases, this study provides a new insight into the immunotoxicity mechanism of PCBs.