表达区间及表达系统（Source）

Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

<1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE

             活性（Activity）

ELISA Data: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA 

SPR Data: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200)

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观（Physical Appearance）

白色冻干粉

标签(Tag)

C-His