产品说明书
FAQ
COA
已发表文献
性能参数
|
表达区间及表达系统（Source） |
Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp27-Cys554.[Accession | EHH53214.1] |
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分子量大小（Molecular Weight） |
The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS- PAGE result. |
|
内毒素（Endotoxin） |
<1EU per μg by the LAL method. |
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纯度（Purity） |
> 95% as determined by SDS- PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.. Bioactivity:Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is > 10000 pmol/min/μg. |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
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重构方法（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230625
