产品说明书
FAQ
COA
已发表文献
核因子kB (NF-kB)是一种序列特异性dna结合蛋白复合体，调节包括人类免疫缺陷病毒在内的病毒基因组和各种细胞基因的表达，特别是参与免疫和炎症反应的基因。在哺乳动物细胞中，NF-kB家族成员包括原癌基因c-Rel、p50/p105 (NFkB1)、p65 (RelA)、p52/p100 (NFkB2)和RelB。所有这些蛋白都有一个保守的300个氨基酸区域，称为Rel同源结构域，该区域负责与DNA结合、二聚和NF-kB的核易位。
产品性质
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别名 |
NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB |
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SwissProt ID |
Q04206 |
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基因ID |
5970 |
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免疫原 |
Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFκB-p65. |
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缓冲液 |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
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分子量 |
Calculated MW: 60 kDa; Observed MW: 65 kDa |
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克隆类型 |
Polyclonal Antibody |
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亚型 |
Rabbit IgG |
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宿主 |
Rabbit |
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物种反应 |
Human,Mouse,Rat |
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推荐应用 |
WB,IHC-P,ICC/IF |
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纯化方式 |
Affinity Chromatography |
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细胞定位 |
细胞质,细胞核,胞质 |
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研究领域 |
内分泌代谢、表观遗传学与核信号、细胞生物学与发育生物学、免疫学与验证 |
应用
稀释比WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
运输与保存方法
冰袋运输，-20℃保存。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
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Western blot |
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Western blot analysis of Phospho-NFκB p65 (Ser536) in HepG2 and HL-60 lysates untreated or treated with TNF using NFκB p65 (Phospho-Ser536) antibody.
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IHC |
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IHC analysis of paraffin-embedded Human breast carcinoma tissue using NFκB p65 (Phospho-Ser536) antibody (left) or the same antibody preincubated with blocking temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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IHC analysis of paraffin embedded Human breast carcinoma tissue using Phospho-NF-KB p65 (Ser536) antibody.High pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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IHC analysis of paraffin embedded Human Lung carcinoma tissue using NFκB (Phospho-Ser536) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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HB220811
