NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.

稀释比 WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

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Western blot

	Western blot analysis of Phospho-NFκB p65 (Ser536) in HepG2 and HL-60 lysates untreated or treated with TNF using NFκB p65 (Phospho-Ser536) antibody.
IHC
	IHC analysis of paraffin-embedded Human breast carcinoma tissue using NFκB p65 (Phospho-Ser536) antibody (left) or the same antibody preincubated with blocking temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	IHC analysis of paraffin embedded Human breast carcinoma tissue using Phospho-NF-KB p65 (Ser536) antibody.High pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	IHC analysis of paraffin embedded Human Lung carcinoma tissue using NFκB (Phospho-Ser536) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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