RSPO2 (R-spondin 2) is a secreted protein that plays a crucial role in regulating Wnt/β-catenin signaling, a pathway essential for embryonic development, tissue homeostasis, and stem cell maintenance. RSPO2 is highly conserved across species and is known for its ability to potentiate Wnt signaling by interacting with LGR5 and LGR4 receptors.RSPO2 also plays a role in intestinal organoid cultures. It has been shown to support the growth and differentiation of intestinal stem cells by enhancing Wnt signaling. This is particularly important for maintaining the long-term stability and functionality of intestinal organoids, which are used to study gastrointestinal diseases and develop personalized therapies. In lung organoid cultures, RSPO2 has been shown to enhance the colony-forming efficiency of club cells, a type of progenitor cell in the lung. RSPO2 activates canonical Wnt/β-catenin signaling, which is crucial for the maintenance and expansion of these cells.

高活性、高纯度、低内毒素、高批间一致性

                                    SDS-PAGE

2 μg/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.

                                      SEC-HPLC

The purity of  Human RSPO2 is greater than 95% as determined by SEC-HPLC.

                                      Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 20ng/mL in the presence of 5ng/mL Recombinant Human Wnt Surrogate.

-25 ~ -15℃保存，收到货之后有效期1年。复溶后， 2 ~ 8 °C保存，7 天有效期。复溶后， -85 ~ -65℃保存，3个月有效期。建议首次使用时将蛋白等分，避免重复冻融循环。