分子生物学
IVD分子诊断
细胞培养与分析
蛋白研究
重组蛋白
细胞因子
抗体
高通量测序建库
病原检测UCF系列
生物医药
工具酶
抑制剂激活剂与常用试剂
仪器
耗材
Recombinant Human EGF Protein,hFc Tag
产品详情
FAQ
产品文档
已发表文献
产品介绍

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors . The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

产品性质
产品特色

高活性、高纯度、低内毒素、高批间一致性

应用案例




                                        SDS-PAGE


                     


Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.



                                                   HPLC


         


The purity of Human EGF is greater than 95% as determined by SEC-HPLC.



                                                   ELISA Data


         


Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.



                                                 SPR Data


         


Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay.



                                         Cell Based Assay


         


Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL.

存储条件

-25~-15℃保存,收到货之日起,有效期1年。复溶后, -85~-65℃保存,3个月有效期。避免反复冻融。

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