Recombinant Biotinylated Human LAG3/CD223 Protein,His-Avi Tag

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产品说明书

FAQ

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已发表文献

 

性能参数

分子别名(Synonyms)

LAG3; FDC;CD223; sLAG-3

表达区间及表达系统(Source)

Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu23-Gly434.[Accession | P18627-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Anti-LAG3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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