Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein,His-Avi Tag

更多活动更多优惠,尽在翌圣商城》

批量采购, 请点击询价

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

GFR alpha-like; GFRAL; GRAL; C6orf144

表达区间及表达系统(Source)

Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser19-Glu351.[Accession | Q6UXV0]

分子量大小(Molecular Weight)

The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE and HPLC

活性(Activity)

ELISA Data: Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 89.1ng/ml determined by ELISA 

Blocking Data: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

  1. Please operate with lab coats and disposable gloves,for your safety.      
  2. This product is for research use only.  

 

 

Ver. CN20231129

400-6111-883