重组蛋白A/G/L是一种基因工程蛋白,蛋白 A/G/L结合了所有蛋白A、蛋白G 和蛋白L的IgG 结合谱,是一种基因融合产物。其含有蛋白A的5个IgG结合区、蛋白G的2个区和蛋白L的5个结合区,细胞壁结合区、细胞膜结合区和白蛋白结合区已从重组蛋白A/G/L中去除,以确保最大的特异性IgG结合。重组蛋白 A/G/L 是纯化多克隆或单克隆 IgG 抗体的理想选择。蛋白A / G / L与人,小鼠,大鼠牛,山羊,绵羊,兔,豚鼠,猪,狗和猫结合IgG。
产品性质
来源 |
E.coli |
分子量 |
Approximately 89.3 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 806 amino acids. |
氨基酸序列 |
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEE PRARPGSGSG KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAGC |
280 nm 处1个单位DO的浓度 |
1.412 mg/ml |
外观 |
无菌过滤的白色冻干粉末 |
纯度 |
> 95% by SDS-PAGE and HPLC analyses |
内毒素水平 |
< 0.1 EU/μg of protein by the LAL method |
配方 |
无添加剂,冻干 |
复溶 |
蒸馏水或盐水溶解 |
运输与保存方法
冰袋运输。-20℃保存,一年有效期。建议第一次使用时分装冻存,避免反复冻融。
注意事项
附录1. 蛋白A、G和L对不同物种Ig的结合能力总表
种属 |
|
Protein A |
Protein G |
Protein L |
Protein A/G |
Protein A/G/L |
人 |
lgG |
+++ |
+++ |
+++ |
+++ |
+++ |
lgG1 |
++++ |
++++ |
++++ |
++++ |
++++ |
|
lgG2 |
++++ |
++++ |
++++ |
++++ |
++++ |
|
lgG3 |
|
+++ |
+++ |
+++ |
+++ |
|
lgG4 |
++++ |
++++ |
++++ |
++++ |
++++ |
|
lgA |
+ |
|
+++ |
+ |
+++ |
|
lgA1 |
+ |
|
+++ |
+ |
+++ |
|
lgA2 |
+ |
|
+++ |
+ |
+++ |
|
lgD |
|
|
+++ |
|
+++ |
|
lgE |
++ |
|
+++ |
|
+++ |
|
lgM |
+ |
|
+++ |
|
+++ |
|
Fab |
+ |
|
+++ |
|
+++ |
|
ScFv |
+ |
|
+++ |
|
+++ |
|
小鼠 |
lgG |
++ |
++ |
+++ |
+++ |
+++ |
lgG1 |
+ |
++++ |
+++ |
+++ |
++++ |
|
lgG2a |
++++ |
++++ |
+++ |
++++ |
++++ |
|
lgG2b |
+++ |
+++ |
+++ |
+++ |
+++ |
|
lgG3 |
++ |
+++ |
+++ |
+++ |
+++ |
|
lgM |
|
|
+++ |
|
+++ |
|
大鼠 |
lgG |
+ |
++ |
+++ |
++ |
+++ |
lgG1 |
|
+ |
+++ |
++ |
+++ |
|
lgG2a |
|
++++ |
+++ |
+++ |
+++ |
|
lgG2b |
|
++ |
+++ |
+ |
++++ |
|
lgG2c |
++ |
++ |
+++ |
+++ |
+++ |
|
lgG3 |
+ |
++ |
? |
++ |
++ |
|
马 |
lgG |
++ |
++++ |
? |
++++ |
++++ |
lgG(ab) |
+ |
|
? |
+ |
+ |
|
lgG(c) |
+ |
|
? |
+ |
+ |
|
lgG(T) |
|
+++ |
? |
+++ |
+++ |
|
山羊 |
lgG |
+ |
++ |
|
+++ |
+++ |
lgG1 |
+ |
+++ |
|
+++ |
+++ |
|
lgG2 |
+++ |
+++ |
|
+++ |
+++ |
种属 |
|
Protein A |
Protein G |
Protein L |
Protein A/G |
Protein A/G/L |
豚鼠 |
lgG |
+++ |
+ |
? |
+++ |
+++ |
lgG1 |
++ |
+ |
? |
++ |
++ |
|
lgG2 |
++ |
+ |
? |
++ |
++ |
|
绵羊 |
lgG |
+ |
++ |
|
+++ |
+++ |
lgG1 |
+ |
++ |
|
+++ |
+++ |
|
lgG2 |
+++ |
+++ |
|
+++ |
+++ |
|
牛 |
lgG |
+ |
+++ |
|
+++ |
+++ |
lgG1 |
+ |
+++ |
|
+++ |
+++ |
|
lgG2 |
+++ |
+++ |
|
+++ |
+++ |
|
兔子 |
lgG |
+++ |
+++ |
+ |
+++ |
+++ |
猫 |
lgG |
+++ |
+ |
? |
+ |
+++ |
狗 |
lgG |
++ |
+ |
? |
+++ |
+++ |
猪 |
lgG |
+++ |
++ |
+++ |
+++ |
+++ |
猴子 |
lgG |
++++ |
++++ |
? |
++++ |
++++ |
仓鼠 |
lgG |
+ |
++ |
+++ |
++ |
++ |
驴 |
lgG |
++ |
+++ |
? |
+++ |
+++ |
HB230208
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