The HA tag is derived from the human influenza hemagglutinin corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. This tag facilitates the detection, isolation, and purification of the proteins.

推荐稀释比 WB:1/5000; IP:1/-1/200; IF:1/1000; ELISA:1/5000-1/20000

0.5ug HA fusion protein+ Primary antibody dilution at 1:10000

Immunofluorescence analysis of HA Tag (9A3) in 293 cells transfected with a HAtag protein using HA Tag (9A3) antibody(red),and DAPI (blue).

Immunoprecipitation analysis of HA Tag (9A3) in 1、untransfected 293 cell lysates2、transfected 293 cell lysates with HAtag fusion protein3、IP (transfected 293+ normal mouse IgG+Protein G agarose)4、IP (transfected 293+antiHA mAb+ Protein G agarose)

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.