Swiss-Prot Acc.Q963I9.Green fluorescent protein (GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
推荐稀释比 WB:1/1000-1/10000;IP:1/20
1) Input (control) 2) IP products, antibody dilution 1:200
Western blot analysis of GFPtag in CHO-K1 transfected by different GFPfusion proteins(A, B, C) or nontransfected (D) cell lysates using GFPtag antibody.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.