翌圣生物 Yeasen｜生命科学试剂与诊断原料一站式平台

Abstract

Preweaning diarrhea and intestinal inflammation represent leading causes of morbidity and economic loss in modern dairy calf production systems, causing impaired growth performance, increased mortality, and substantial veterinary costs that collectively constrain farm profitability. Faecalibacterium duncaniae, a key butyrate-producing commensal bacterium, is consistently depleted in diarrheic dairy calves, yet its protective effects on the ruminant intestinal epithelium remain mechanistically understood. In this study, a long-term expandable ruminant colonic organoid model was used to investigate the effects of F. duncaniae-derived metabolites under inflammatory conditions induced by tumor necrosis factor-α (TNF-α). TNF-α stimulation caused epithelial injury characterized by elevated inflammatory cytokine expression, impaired barrier function, and increased epithelial permeability. Treatment with F. duncaniae cell-free supernatant markedly attenuated inflammatory responses, restored epithelial proliferation, and preserved tight junction integrity, as evidenced by reduced permeability and increased expression of barrier-related proteins. Transcriptomic analysis further indicated suppression of inflammation-associated signaling pathways, including chemokine signaling and NF-κB pathways, accompanied by enrichment of energy metabolism and epithelial repair pathways, thereby creating a molecular environment conducive to epithelial barrier improvement and repair. In addition, comparison with the bacterial culture medium and the single metabolite sodium butyrate (NaB) further supported the role of F. duncaniae-derived metabolites in barrier repair. Collectively, these findings demonstrate that F. duncaniae-derived metabolites protect intestinal epithelial integrity under inflammatory stress and provide mechanistic and translational support for microbiota-targeted nutritional strategies to improve gut health in dairy calves, thereby establishing a foundation for the develo