链霉亲和素是一种来源于阿维丁链霉菌(streptomyces avidinii)的生物素结合蛋白,其非特异性结合远比亲和素低,且与蛋清亲和素在中性pH值具有净正电荷和包含大约7%的碳水化合物相比,链霉亲和素蛋白具有在中性pH值几乎没有净电荷,不含有碳水化合物等更有利的化学性质,所以它被广泛用来作为抗生物素蛋白的替代品。
本品是YSFluorTM 488标记链霉亲和素,该荧光素YSFluorTM 488,Amax(最大激发波长)为493 nm,Emax(最大发射波长)为519 nm,同Alex Fluor 488和FITC的光谱性质一样,通常可考虑相互替代。建议工作液浓度:0.5-2 µg/mL(For most applications)。
链霉亲和素浓度(Streptavidin Concentration) | 0.9 mg/mL |
缓冲液(Buffer) | 0.005 M磷酸钠,0.125 M氯化钠,pH 7.6 |
稳定剂(Stabilizer) | 7.5 mg/mL BSA(无IgG,无蛋白酶) |
荧光基团(Fluorophore) | YSFluorTM 488,Ex/Em=493 nm/519 nm |
防腐剂(Preservative) | 0.025% 叠氮化钠 |
-25 ~ -15℃保存,有效期1年,尽量避免反复冻融。
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