Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

推荐稀释比 WB:1/1000-1/10000; IP:1/500

Western blot analysis of HA Tag (3C6) in CHO-K1 (D) or CHO-K1 transfected with different Flagfusion proteins (A, B, C), using Flagtag antibody.

Western blot analysis of Flag Tag (10E1) in CHO-K1 cells transfected with FlagGADD45α (C and D), 3*FlagGADD45α (A and B) as indicated, using Flagtag antibody (left) and GADD45α antibody (201061, right) as loading control.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.