The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Western

Western blot analysis of Atg7(Apg7) in (1) HepG2 lysates; (2) mouse spleen lysates; (3) rat kidney lysates using ATG7 antibody.