Ampicillin，中文名氨苄青霉素，同义名氨苄西林，一种β-内酰胺类抗生素，属于青霉素家族的一员，同样敏感于β-内酰胺酶，后者可裂解氨苄西林的β-内酰胺环。Ampicillin广谱抑制革兰氏阳性菌，阴性菌以及厌氧菌，其抑菌机制在于：干扰青霉素结合蛋白（penicillin binding protein，PBP）活性，PBP参与肽聚糖合成的最后一步，其可催化丙氨酸和赖氨酸残基间形成五甘氨酸交联，此交联维持细胞壁的完整性，并维持细菌的正常生长。另外，Ampicillin仅对生长的大肠杆菌（Escherichia col）具有杀菌作用。

Ampicillin普遍用作细胞培养中的一种选择性抗生素，用于配制含氨苄的LB或LB平板等，也可用于培养细胞的杀菌。Ampicillin抑制革兰氏阴性和革兰氏阳性细菌，最小抑制浓度（MIC）分别为0.03-3 μg/mL和0.02-1.5 mg/mL。高压灭菌的琼脂或培养基需冷却到45-50℃再加入氨苄青霉素溶液，含氨苄青霉素的平板在2-8℃可保存2周。在37°C培养条件下，氨苄青霉素可稳定3天。

本品为氨苄青霉素钠盐，细胞筛选常用的浓度为50-100 μg/mL。

其他抑菌剂和抗生素选购请参考抑菌剂-选购指南和稳定高品质抗生素的相关专题。

本产品是一种广谱半合成青霉素类抗生素，在常温下稳定，但对酸和β-内酰胺酶不稳定。

研究应用：

1. 微生物学研究：用于研究细菌耐药机制和抗菌药物的抗菌活性。
2. 药物开发：作为模型药物研究药物代谢、药代动力学和药物相互作用。
3. 分子生物学：常用于分子克隆实验中，作为选择标记用于筛选转化子。

相关产品推荐：

冰袋运输。2-8℃干燥保存，有效期3年。

Q：一般的建议使用浓度是多少？

A：本品为氨苄青霉素钠盐，细胞筛选常用的浓度为 50-100 μg/mL。

Q：产品的纯度是多少呢？

A：纯度是91%-100.5%，具体的参考购买批次的COA

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